Expression, purification and spectrophotometric analysis of nucleoside hydrolase from Leishmania chagasi (LcNH)

We present here for the first time, the expression and purification protocols to obtain the enzymes LcNH1 and LcNH2 that can be employed to explore novel strategies to produce nucleoside hydrolase inhibitors for use in chemotherapy. Protein integrity was also confirmed by SDS-PAGE gel, mass spectrometry and enzymatic activity.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research