Genetic disruption of guanylyl cyclase/natriuretic peptide receptor-A upregulates renal (pro) renin receptor expression in Npr1 null mutant mice

The objective of the present study was to determine whether targeted-disruption of Npr1 gene (encoding for guanylyl cyclase/natriuretic peptide receptor-A; GC-A/NPRA) upregulates pro(renin) receptor (P)RR expression and leads to the activation of MAPKs in Npr1 gene-knockout mice. The Npr1 homozygous (Npr1−/−; 0-copy), heterozygous (Npr1+/−; 1-copy), wild-type (Npr1+/+; 2-copy), and gene-duplicated (Npr1++/++; 4-copy) mice were utilized. To identify the canonical pathway of (P)RR, we administered ACE-1 inhibitor (captopril), AT1R blocker (losartan), and MAPKs inhibitors (U0126 and SB203580) to all Npr1 mice genotypes. The renal expression of (P)RR mRNA was increased by 3-fold in 0-copy mice and 2-fold in 1-copy mice compared with 2-copy mice, which was also associated with significantly increased expression of ACE-1 and AT1R mRNA levels. Similarly, the phosphorylation of MAPKs (Erk1/2 and p-p38) was enhanced by 3.5-fold and 3.2-fold, respectively, in 0-copy mice with significant increases in 1-copy mice compared with 2-copy mice. The kidney and plasma levels of proinflammatory cytokines were significantly elevated in 0-copy and 1-copy mice. Treatment with captopril and losartan did not alter the expression of (P)RR in any of the Npr1 mice genotypes. Interestingly, losartan significantly reduced the phosphorylation of Erk1/2 and p38 in Npr1 mice. The present results suggest that the ablation of Npr1 upregulates (P)RR, MAPKs (Erk1/2 and p38), and proinflammator...
Source: Peptides - Category: Biochemistry Source Type: research

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ConclusionsThe use of adhesive can improve masticatory performance of new complete dentures users with normal mandibular ridges in the initial adaptation period up to 60 days after insertion. For resorbed ridges subjects, the use of adhesive had a negative impact on masticatory discomfort/disability OHRQoL at 30 days after insertion.
Source: Journal of Prosthodontic Research - Category: Dentistry Source Type: research
ConclusionEATP lowered the serum uric acid level and increased the urine uric acid level through excretion, which is useful in the treatment of gout. Hence the EATP was found to be helpful in the treatment of gout.
Source: Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Publication date: Available online 21 August 2019Source: Journal of Ginseng ResearchAuthor(s): Moon Young Park, Se Jik Han, Donggerami Moon, Sangwoo Kwon, Jin-Woo Lee, Kyung Sook KimAbstractBackgroundRed ginseng contains components that can be used in skincare to delay the aging process, including microelements, vitamins, essential oils, and fatty acids. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis.MethodsHuman dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using at...
Source: Journal of Ginseng Research - Category: Complementary Medicine Source Type: research
ConclusionDifferentially expressed in tumors, PLA2G4A, PRKCB, PIK3R1, KDR, PLA2G1B and PTGS2 are potential therapeutic targets of Yupingfengsan in the treatment of LAD, and MAPK, VEGF and ErbB signaling pathways are involved.
Source: European Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Publication date: Available online 22 August 2019Source: Journal of Cardiology CasesAuthor(s): Shohei Kataoka, Ken Kato, Hiroyuki Tanaka, Tamotsu TejimaAbstractIt is challenging to perform ablation of ventricular tachycardia (VT) from the left ventricle (LV) in patients without catheter access to the LV. A 50-year-old man was referred to our hospital for VT. He underwent mechanical aortic and mitral valve replacement for infective endocarditis and embolic myocardial infarction in the left ventricular inferior wall during a surgery. Anti-arrhythmia drugs (AADs) such as sotalol and bisoprolol were initiated and implantable c...
Source: Journal of Cardiology Cases - Category: Cardiology Source Type: research
Publication date: Available online 22 August 2019Source: Journal of Cardiology CasesAuthor(s): Takashi Ando, Hiroshi Okada, Makoto Takeda, Tomio UmemotoAbstractPeriaortitis is one of the less common manifestations of the aorta pathology. Periaortitis mostly arises from the outer layers of the abdominal aorta and iliac arteries. However, other large arteries may also be involved, but rarely the thoracic aorta. Here we present a successful conservative treatment using prednisolone therapy for periaortitis of the aortic arch which shows various clinical symptoms.
Source: Journal of Cardiology Cases - Category: Cardiology Source Type: research
Publication date: October 2019Source: Interventional Cardiology Clinics, Volume 8, Issue 4Author(s): Julian Yeoh, Jonathan Hill
Source: Interventional Cardiology Clinics - Category: Cardiology Source Type: research
Publication date: October 2019Source: Interventional Cardiology Clinics, Volume 8, Issue 4Author(s): David A. Power, Paul Guedeney, George D. Dangas
Source: Interventional Cardiology Clinics - Category: Cardiology Source Type: research
Publication date: October 2019Source: Interventional Cardiology Clinics, Volume 8, Issue 4Author(s):
Source: Interventional Cardiology Clinics - Category: Cardiology Source Type: research
Publication date: October 2019Source: Interventional Cardiology Clinics, Volume 8, Issue 4Author(s):
Source: Interventional Cardiology Clinics - Category: Cardiology Source Type: research
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