Biodegradation potential of polycyclic aromatic hydrocarbons by immobilized Klebsiella sp. in soil washing effluent.

Biodegradation potential of polycyclic aromatic hydrocarbons by immobilized Klebsiella sp. in soil washing effluent. Chemosphere. 2019 May;223:140-147 Authors: Xu X, Zhou H, Chen X, Wang B, Jin Z, Ji F Abstract A strain KL (Klebsiella sp.), with a high polycyclic aromatic hydrocarbons (PAHs) degradation efficiency, was isolated and purified. Immobilization of strain KL using a boric acid-CaCl2 cross-linking method based on polyvinyl alcohol (PVA)-sodium alginate (SA)-nano alumina (ALNPs) composite was investigated for removal of phenanthrene (PHE), fluoranthene (FLA), and pyrene (PYR) in soil washing effluent. The concentration of PVA, SA, and ALNPs in immobilized beads had significant effects on the physicochemical properties and biodegradation performance. When beads had a PVA, SA, and ALNPs content of 10% (w/v), 0.8% (w/v), and 0.7% (w/v), and the initial biomass dosage was 10% (v/v), the biodegradation efficiency and mass transfer performance of the immobilized beads were optimal with the specific surface area of 13.3971 m2/g. Scanning electron microscopy (SEM) showed that the surface of immobilized beads was dense. The growth and adhesion of cells inside the beads were adequate, and pores of the beads were abundant and irregularly staggered. The immobilization method was successfully applied to the treatment of the three PAHs in soil washing effluent. Adsorption of beads contributed to PAHs removal in the initial stage of degr...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research