Quantitative analysis for lipophilic drug transport through a model lipid membrane with membrane retention.

In this study, we developed a permeability assay for lipophilic drugs with poor water solubility using a freestanding lipid bilayer. The lipid bilayer was created within a UV cuvette, and the number of transported molecules through the bilayer was estimated by measuring the UV absorbance over time. We then measured the permeability of six tested compounds, and there was a significant difference in permeability between the Biopharmaceutics Classification System (BCS) class 2 and class 4 compounds. In addition, the fraction of molecules trapped in the lipid bilayer were estimated for each compound as well. As a result, the drugs with higher lipophilicity were found to undergo more membrane retention, which agrees with the previous reports, supporting that our model lipid membrane system could also be applied to investigate the drug-lipid interaction. PMID: 31022441 [PubMed - as supplied by publisher]
Source: European Journal of Pharmaceutical Sciences - Category: Drugs & Pharmacology Authors: Tags: Eur J Pharm Sci Source Type: research