A Pertussis Outer Membrane Vesicle-Based Vaccine Induces Lung-Resident Memory CD4 T Cells and Protection Against Bordetella pertussis, Including Pertactin Deficient Strains

In this study we have evaluated whether our OMVs vaccine is capable of overcoming the deficiencies of commercial vaccines in both controlling infections caused by PRN(-) isolate/strain and inducing memory immunity. We found that our OMVs-based formulation has a higher protective capacity against the PRN(-) bacteria than that induced with a commercial aP vaccine. We found that CD4 T cells with a tissue-resident memory (TRM) cell phenotype (CD44+CD62LlowCD69+ and/or CD103+) accumulated in the lungs of mice after the second OMVs vaccine immunization. CD4 TRM cells were also detected in mice immunized with wP vaccine, but not in the animals immunized with a commercial aP vaccine. The CD4 TRM cell population was significantly expanded through local proliferation following respiratory challenge of mice with B. pertussis. These CD4 TRM cells secreted IFN-γ and IL-17 that have previously been shown to play a critical role in adaptive immunity against B. pertussis infection. Our findings suggest that the OMVs-vaccine is an ideal candidate for the development of a third generation pertussis vaccine. Materials and Methods Animals C57BL/6 (8-week-old) mice were obtained from Harlan Laboratories U.K. or the Comparative Medicine Unit, Trinity College Dublin and housed in a specific pathogen–free facility. All animal experiments performed in Dublin were conducted in accordance with the recommendations and guidelines and under licenses approved by the Health Products Regulator...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 18 July 2019Source: Small Ruminant ResearchAuthor(s): J.M. González, T. Navarro, D. Lacasta, J.J. Ramos, L.M. Ferrer, N.G.C. Vasileiou, M. de las HerasABSTRACTVaccination programs against Ovine Respiratory Complex (ORC) in lambs should be accompanied by non-specific preventive measure, e.g., establishment of proper management and husbandry conditions. Implementing a vaccination plan against ORC requires: involvement of all farm staff, recording data, a positive cost:benefit analysis and continuous monitoring over time. Licensed vaccines for sheep are only against Mannheimia haemoly...
Source: Small Ruminant Research - Category: Zoology Source Type: research
Publication date: Available online 19 July 2019Source: Veterinary ParasitologyAuthor(s): Juan Pablo Ek-Huchim, Isabel Jiménez-García, Rossanna Rodríguez-CanulAbstractMonogeneans Gyrodactylus von Nordmann 1832, cause outbreaks of gyrodactylosis in aquaculture settings worldwide. Detection of Gyrodactylus spp. is based on the morphological identification of isolated parasites after fish necropsy. Contributing to the diagnosis of gyrodactylosis, in this study, a non-destructive PCR assay was standardized; the PCR was first performed using genomic DNA of Gyrodactylus spp. isolated from the surface of the N...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Publication date: Available online 19 July 2019Source: Veterinary MicrobiologyAuthor(s): Milan Obradovic, J. Alex Pasternak, Siew Hon Ng, Brenda Allan, Robert Brownlie, Heather L. WilsonAbstractLawsonia intracellularis is an obligate intracellular microorganism and the causative agent of porcine proliferative enteropathy. Due to its obligate intracellular nature, characterization of antigens and proteins involved in host-pathogen interaction and immune recognition have been difficult to achieve using conventional microbiological techniques. In this work, we used 2-dimensional gel electrophoresis coupled with Western-immuno...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, we describe the results of surveillance for psittacid herpesviruses (PsHVs), psittacine adenovirus (PsAdV), BFDV and C. psittaci in wild cacatuids in Victoria, Australia. Samples were collected from 55 birds of four species, and tested using genus or family-wide polymerase chain reaction methods coupled with sequencing and phylogenetic analyses for detection and identification of known and novel pathogens. There were no clinically observed signs of illness in most of the live birds in this study (96.3%; n = 53). Beak and Feather Disease Virus was detected with a prevalence of 69.6% (95% CI 55.2...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
CONCLUSIONS: On the basis of the study, it was indicated that the group of the highest risk are students of junior secondary school, probably because of the great interest in physical attractiveness in this period of life, as well as individuals with higher BMI. We believe that for more effective diagnosis it would be advisable to adopt a cut-off point for orthorexia in the ORTO-15 at the level of 35 points, as postulated by Stochel, Janas-Kozik et al. The 40-point threshold is associated with considerable overdiagnosis of the phenomenon. PMID: 31317965 [PubMed - in process]
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
CONCLUSIONS: Orthorexia nervosa is not a separate clinical entity. It does not belong to the OCD spectrum, but meets the criteria of eating disorder spectrum. PMID: 31317964 [PubMed - in process]
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
CONCLUSIONS: 1) More than . of studied adolescents realize that there is no integrated system of suicide prevention; 2) The family, according to high school students, plays the greatest role in preventing self-destructive behaviors; 3) There is a need of greater involvement of schools, the Church, the police, health care institutions and the media in education and raising adolescents' awareness in the field of self-destructive behaviors.`. PMID: 31317963 [PubMed - in process]
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
This article presents recommendations of the Polish Psychiatric Association regarding approach to pregnancy loss and unsuccessful in vitro treatment of infertility. From the psychological perspective pregnancy loss and perinatal death are amongst the most stressful events in human life - carrying increased risk of developing affective, anxiety or post-traumatic stress disorders. Psychologists, physicians and the rest of the medical staff should provide integrated and individualized care which should be based on respect, empathy and expertise. The necessary phases of support for women experiencing pregnancy loss are: (1) ph...
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
Authors: Rybakowski J, Cubała WJ, Gałecki P, Rymaszewska J, Samochowiec J, Szulc A, Dudek D Abstract In the article, the recommendations of the Polish Psychiatric Association regarding pharmacological treatment of women with bipolar disorder during pregnancy and postpartum period were presented. The issue pertains to every twentieth woman wanting to get pregnant. Before planned pregnancy, it is advisable to obtain a several-month stabilization of psychiatric state, to establish treatment with one mood-stabilizing drug (except for valproate and carbamazepine) or gradual discontinuation of drugs in case of mild cou...
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
Authors: Samochowiec J, Rybakowski J, Galecki P, Szulc A, Rymaszewska J, Cubiała WJ, Dudek D Abstract Treatment of depressive disorders in women of childbearing age requires special attention due to the possibility of planned or unplanned pregnancy and the specificity of mood disorders associated with the perinatal period. A doctor who treats depression in a woman of childbearing age should openly discuss with the patient her sexuality and the possibility of becoming pregnant. A psychiatrist may encounter various problems, such as: a therapeutic decision regarding a woman suffering from recurrent depression who re...
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
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