A Pertussis Outer Membrane Vesicle-Based Vaccine Induces Lung-Resident Memory CD4 T Cells and Protection Against Bordetella pertussis, Including Pertactin Deficient Strains

In this study we have evaluated whether our OMVs vaccine is capable of overcoming the deficiencies of commercial vaccines in both controlling infections caused by PRN(-) isolate/strain and inducing memory immunity. We found that our OMVs-based formulation has a higher protective capacity against the PRN(-) bacteria than that induced with a commercial aP vaccine. We found that CD4 T cells with a tissue-resident memory (TRM) cell phenotype (CD44+CD62LlowCD69+ and/or CD103+) accumulated in the lungs of mice after the second OMVs vaccine immunization. CD4 TRM cells were also detected in mice immunized with wP vaccine, but not in the animals immunized with a commercial aP vaccine. The CD4 TRM cell population was significantly expanded through local proliferation following respiratory challenge of mice with B. pertussis. These CD4 TRM cells secreted IFN-γ and IL-17 that have previously been shown to play a critical role in adaptive immunity against B. pertussis infection. Our findings suggest that the OMVs-vaccine is an ideal candidate for the development of a third generation pertussis vaccine. Materials and Methods Animals C57BL/6 (8-week-old) mice were obtained from Harlan Laboratories U.K. or the Comparative Medicine Unit, Trinity College Dublin and housed in a specific pathogen–free facility. All animal experiments performed in Dublin were conducted in accordance with the recommendations and guidelines and under licenses approved by the Health Products Regulator...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Source: Genomics Proteomics ... - Category: Genetics & Stem Cells Authors: Tags: Front Microbiol Source Type: research
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Source: Respiratory Care - Category: Respiratory Medicine Authors: Tags: Semin Respir Crit Care Med Source Type: research
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Source: Respiratory Care - Category: Respiratory Medicine Authors: Tags: Semin Respir Crit Care Med Source Type: research
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Source: EurekAlert! - Biology - Category: Biology Source Type: news
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Source: Guardian Unlimited Science - Category: Science Authors: Tags: Coronavirus outbreak World news UK news US news Australia news Science Infectious diseases Microbiology Medical research Source Type: news
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Source: The Journal of Vascular Access - Category: Surgery Tags: J Vasc Access Source Type: research
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Source: Guardian Unlimited Science - Category: Science Authors: Tags: New Zealand Coronavirus outbreak Asia Pacific Infectious diseases Science Source Type: news
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