T929I and K1774N mutation pair and M918L single mutation identified in the voltage-gated sodium channel gene of pyrethroid-resistant Thrips tabaci (Thysanoptera: Thripidae) in Japan

Publication date: Available online 25 April 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Akiya Jouraku, Seigo Kuwazaki, Hiroyuki Iida, Izumi Ohta, Hisao Kusano, Motonori Takagi, Tomoya Yokoyama, Naoya Kubota, Manabu Shibao, Kanako Shirotsuka, Akeo Iwasaki, Yuji Takezawa, Mitsuyoshi TakedaAbstractPyrethroid-resistance in onion thrips, Thrips tabaci, has been reported in many countries including Japan. Identifying factors of the resistance is important to correctly monitoring the resistance in field populations. To identify pyrethroid-resistance related genes in T. tabaci in Japan, we performed RNA-Seq analysis of seven T. tabaci strains including two pyrethroid-resistant and five pyrethroid-susceptible strains. We identified a pair of single point mutations, T929I and K1774N, introducing two amino acid mutations, in the voltage-gated sodium channel gene, a pyrethroid target gene, in the two resistant strains. The K1774N is a newly identified mutation located in the fourth repeat domain of the sodium channel. Genotyping analysis of field-collected populations showed that most of the T. tabaci individuals in resistant populations carried the mutation pair, indicating that the mutation pair is closely associated with pyrethroid-resistance in Japan. Another resistance-related mutation, M918L, was also identified in part of the resistant populations. Most of the individuals with the mutation pair were arrhenotokous while all individuals with the M918L single mutation...
Source: Pesticide Biochemistry and Physiology - Category: Biochemistry Source Type: research