Evaluation of Plasma Extracellular Vesicle MicroRNA Signatures for Lung Adenocarcinoma and Granuloma With Monte-Carlo Feature Selection Method

In this study, the lung adenocarcinoma patients and granuloma patients were treated as positive samples and negative samples, respectively. We used weighted accuracy to evaluate the RIPPER prediction performance, i.e., the average of the accuracies of positive samples and negative samples. Results The Discriminative MicroRNAs Between Lung Adenocarcinoma and Granuloma Patients in Whole Plasma, EV, and EV-Free Plasma The miRNA expression profiles of lung adenocarcinoma and granuloma patients in whole plasma, EV and EV-free plasma were analyzed separately. In whole plasma, the top 10 discriminative miRNAs were hsa-miR-223-3p, hsa-miR-501-5p, hsa-miR-130b-3p, hsa-miR-5010-5p, hsa-miR-330-5p, hsa-miR-378f, hsa-miR-3158-3p, hsa-miR-542-3p, hsa-miR-183-5p and hsa-miR-942-5p. In EV, the top 10 discriminative miRNAs were hsa-miR-23b-3p, hsa-miR-548ac, hsa-miR-3126-3p, hsa-miR-15b-5p, hsa-miR-205-5p, hsa-miR-5010-5p, hsa-miR-331-5p, hsa-miR-1249-3p, hsa-miR-548c-5p, and hsa-miR-1827. In EV-free plasma, the top 10 discriminative miRNAs were hsa-miR-511-3p, hsa-miR-376a-3p, hsa-miR-3150b-3p, hsa-miR-3150b-5p, hsa-miR-3168, hsa-miR-98-5p, hsa-miR-3136-5p, hsa-miR-210-5p, hsa-miR-340-3p, and hsa-miR-636. Figure 1 shows the heatmaps of the top 10 miRNAs in whole plasma, EV and EV-free plasma. The miRNAs and patients were clustered using ward D2 method (Murtagh and Legendre, 2014) based on Euclidean distance. The R package pheatmap1 was applied to plot the heatmaps. It can be seen from Fi...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research

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This study aimed to investigate the risk of self-harm following diagnosis of different psychiatric disorders in an Asian population, through a review of inpatient records retrieved from the Hong Kong Clinical Data Analysis and Reporting System (CDARS).MethodsFor this nested case-control study, the inpatient data of people admitted for any reason to Hong Kong public hospitals, between Jan 1, 2000, and Dec 31, 2010, were extracted from CDARS. Cases were people aged 10 years or older who had been admitted to inpatient care with a first-listed record of psychiatric disorder during the study period. The seven psychiatric disord...
Source: The Lancet Psychiatry - Category: Psychiatry Source Type: research
In conclusion, the addition of PPIs to the treatment regimen of breast cancer appears to be a promising strategy to potentiate the efficacy of chemotherapy and may suppress cancer metastasis. PMID: 31955147 [PubMed - in process]
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Abstract A series of nine new 2,3-disubstituted 4(3H)-quinazolin-4-one derivatives was furnished starting from the 2-propyl-4(3H)-quinazo-line-4-one (2). The reinvestigation of the key starting quinazolinone 2 was performed under microwave irradiation (MW) and solvent-free conditions. Combination of MW and phase-transfer catalysis using tetrabutylammonium benzoate (TBAB) as a novel neutral ionic catalyst was used for carrying out N-alkylation and condensation reactions of compound 2 as a simple, efficient and eco-friendly technique. The structure of the synthesized compounds was elucidated using different spectral...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
This study aims to unveil the mechanisms underlying the pro-apoptotic effect of Cur-NLC on HepG2 cells. Evidence indicates that low expression of death receptors (DRs) on cancer cell membranes leads to attenuated apoptosis signaling. This study showed that Cur-NLC significantly increased total expression of DR5 protein while simultaneously upregulated cell membrane expression of DR5. Cur-NLC significantly increased caspase-8 and caspase-3 activities, accompanied by increased apoptosis. Furthermore, enhanced apoptosis was inhibited in the presence of a pan-caspase inhibitor, Z-VAD-FMK. Therefore, Cur-NLC induced activation ...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Abstract The incidence of mortality of prostate cancer (PCa) has been an uptrend in recent years. Our previous study showed that the sex-determining region Y-box 7 (SOX7) was low-expressed and served as a tumor suppressor in PCa cells. Here, we describe the effects of polyphyllin D (PD) on proliferation and cell cycle modifications of PCa cells, and whether SOX7 participates in this process. PC-3 cells were cultured in complete medium containing PD for 12, 24, and 48 h. MTT assay was used to investigate the cytotoxic effects of PD. Cell cycle progression was analyzed using propidium iodide (PI) staining, and prote...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Conclusion: SliceTracker is modular and extensible open source platform for supporting image processing aspects of the tpMRgBx procedure. It has been successfully utilized to support clinical research procedures at our site.
Source: IEEE Transactions on Biomedical Engineering - Category: Biomedical Engineering Source Type: research
Publication date: Available online 20 January 2020Source: NeurocomputingAuthor(s): Wei Li, Xuefeng Lin, Xi ChenAbstractApplying machine learning methods to various modality medical images and clinical data for early diagnosis of Alzheimer's disease (AD) and its prodromal stage has got lots of significant results. So far, the image data input to classifier mainly focus on 2D or 3D images. Although some functional imaging technologies, such as functional magnetic resonance imaging (fMRI), generate 4D data which contain both spatial and time-varying information of the brain, for the lack of suitable 4D image processing algori...
Source: Neurocomputing - Category: Neuroscience Source Type: research
Authors: Yoon D, Jang JH, Choi BJ, Kim TY, Han CH Abstract Biosignals like electrocardiogram or photoplethysmogram have been widely used for monitoring and determining status of patients. However, it has been recently discovered that more information than that we have used traditionally were included in the biosignals after artificial intelligence (AI) was applied. Most meaningful advancement of current AI was in deep learning. The deep learning-based models show the best performance in most area in current due to the distinguished characteristic that it is able to extract important features from raw data. For that...
Source: Korean Journal of Anesthesiology - Category: Anesthesiology Tags: Korean J Anesthesiol Source Type: research
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Source: Gut Microbes - Category: Microbiology Authors: Source Type: research
In this study, we examined bacterial communities from cyanobacterial-lichen and moss crust soils in five desert habitats in northern China, and evaluated the relative importance of environmental factors and biocrust development versus geographic distance to the distance–decay relationship. To explore the effects of the sampling scale on geographical patterns, we also examined soil bacterial communities along the slope of sand dunes. Across the five desert habitats, bacterial α-diversity, phylogenetic diversity, and the dominant bacterial phyla in cyanobacterial-lichen crusts did not increase consistently with p...
Source: Soil Biology and Biochemistry - Category: Biology Source Type: research
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