Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice

This study was carried out in accordance with the recommendations of the ethics standards defined by the EU legislation on the use of experimental animals (2010/63/EU) and Czech animal welfare act. The protocol was approved by the Institute of Microbiology animal care and use committee (approval ID: 85/2015 and 108/2016). Diets and Experimental Design In most experiments, shortly after weaning, the mice were switched either to animal protein-based diet—control (aCD, 176 g/kg crude protein; Cat# C1000) and high-protein diet (aHPD, 514 g/kg crude protein; Cat# C1001) or plant protein-based diet—control (pCD, 173 g/kg crude protein; Cat# C 1000/110007) and high-protein diet (pHPD, 500 g/kg crude protein; Cat# C1001/1001127; all from Altromin Spezialfutter, GmbH &Co. KG). All these diets were prepared synthetically, containing either casein (animal) or wheat gluten (plant) as a protein source (Table 1). Mice were maintained on these diets for 3 weeks before the experiment started and then throughout the whole experiment. In some experiments, the long-time effect of the diet was analyzed by switching the diet already to the parental generation of the mice. We measured gut permeability for macromolecules in healthy mice fed with different diets. For this purpose, we treated mice orally with 440 mg/kg of body weight of FITC-labeled 3–5 kD molecule of dextran (Merck, Cat# FD4), and measured serum fluorescence 4 h later, as we published previously (35). TABL...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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