Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice
This study was carried out in accordance with the recommendations of the ethics standards defined by the EU legislation on the use of experimental animals (2010/63/EU) and Czech animal welfare act. The protocol was approved by the Institute of Microbiology animal care and use committee (approval ID: 85/2015 and 108/2016). Diets and Experimental Design In most experiments, shortly after weaning, the mice were switched either to animal protein-based diet—control (aCD, 176 g/kg crude protein; Cat# C1000) and high-protein diet (aHPD, 514 g/kg crude protein; Cat# C1001) or plant protein-based diet—control (pCD, 173 g/kg crude protein; Cat# C 1000/110007) and high-protein diet (pHPD, 500 g/kg crude protein; Cat# C1001/1001127; all from Altromin Spezialfutter, GmbH &Co. KG). All these diets were prepared synthetically, containing either casein (animal) or wheat gluten (plant) as a protein source (Table 1). Mice were maintained on these diets for 3 weeks before the experiment started and then throughout the whole experiment. In some experiments, the long-time effect of the diet was analyzed by switching the diet already to the parental generation of the mice. We measured gut permeability for macromolecules in healthy mice fed with different diets. For this purpose, we treated mice orally with 440 mg/kg of body weight of FITC-labeled 3–5 kD molecule of dextran (Merck, Cat# FD4), and measured serum fluorescence 4 h later, as we published previously (35). TABL...
Publication date: Available online 30 May 2020Source: Life SciencesAuthor(s): Qi Fu, Zhenhai Yu
Publication date: Available online 30 May 2020Source: Life SciencesAuthor(s): Lakshmikripa Jagannathan, Emily Socks, Priya Balasubramanian, Robert McGowan, Thomas M. Herdt, Reza Kianian, Sheba M.J. MohanKumar, P.S. MohanKumar
Publication date: Available online 29 May 2020Source: Life SciencesAuthor(s): Nagesh Kishan Panchal, E.P. Sabina
Publication date: Available online 29 May 2020Source: Carbohydrate PolymersAuthor(s): Juntao Tang, Yang Li, Yang Song, Xinyun Wu, Guipeng Yu, Kam Chiu Tam
Publication date: Available online 29 May 2020Source: Carbohydrate PolymersAuthor(s): Katarzyna Nawrotek, Michał Tylman, Agnieszka Adamus-Włodarczyk, Karolina Rudnicka, Justyna Gatkowska, Marek Wieczorek, Radosław Wach
Publication date: Available online 29 May 2020Source: Carbohydrate PolymersAuthor(s): Noha Hesham Radwan, Maha Nasr, Rania A.H. Ishak, Nourtan F. Abdeltawab, Gehanne A.S. Awad
Abstract Biological denitrification is a promising and green technology for air pollution control. To investigate the nitric oxide reductase (NOR) that dominates NO reduction efficiency in biological purification, the heterologous prokaryotic expression system of the norB gene, which encodes the core peptide of the catalytic reduction structure in the NOR from Achromobacter denitrificans strain TB, was constructed in Escherichia coli BL21 (DE3). Results showed that the 1218 bp-long norB gene was expressed at the highest level under 1.0 mM IPTG for 5 h at 30 °C, and the relative expression a...
In this study, five machine learning algorithms and twelve types of molecular fingerprints were employed to generate QSAR discriminant models for mitochondrial toxicity. A threshold moving method was adopted to resolve the imbalance issue in the training data. Consensus of the models by an averaging probability strategy improved prediction performance. The best model has correct classification rates of 81.8% and 88.3% in ten-fold cross validation and external validation, respectively. Substructures such as phenol, carboxylic acid, nitro and arylchloride were found informative through analysis of information gain and freque...
Abstract To understand the biofilm formation of biofilm-based processes in wastewater treatment plants (WWTPs), the interaction mechanisms between extracted extracellular polymeric substances (EPS) and three model carrier surfaces (i.e., negatively charged hydrophilic silica, positively charged hydrophilic alumina, and neutral charged hydrophobic polystyrene) were investigated employing a laboratory quartz crystal microbalance with dissipation monitoring equipment (QCM-D) and an atomic force microscope (AFM). The data suggested that surface charge and hydrophobicity of both EPS and carriers played significant role...
Publication date: Available online 29 May 2020Source: Wilderness &Environmental MedicineAuthor(s): Brendan H.A. Milliner, Graham Brant-Zawadzki, Scott E. McIntosh
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