Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice

This study was carried out in accordance with the recommendations of the ethics standards defined by the EU legislation on the use of experimental animals (2010/63/EU) and Czech animal welfare act. The protocol was approved by the Institute of Microbiology animal care and use committee (approval ID: 85/2015 and 108/2016). Diets and Experimental Design In most experiments, shortly after weaning, the mice were switched either to animal protein-based diet—control (aCD, 176 g/kg crude protein; Cat# C1000) and high-protein diet (aHPD, 514 g/kg crude protein; Cat# C1001) or plant protein-based diet—control (pCD, 173 g/kg crude protein; Cat# C 1000/110007) and high-protein diet (pHPD, 500 g/kg crude protein; Cat# C1001/1001127; all from Altromin Spezialfutter, GmbH &Co. KG). All these diets were prepared synthetically, containing either casein (animal) or wheat gluten (plant) as a protein source (Table 1). Mice were maintained on these diets for 3 weeks before the experiment started and then throughout the whole experiment. In some experiments, the long-time effect of the diet was analyzed by switching the diet already to the parental generation of the mice. We measured gut permeability for macromolecules in healthy mice fed with different diets. For this purpose, we treated mice orally with 440 mg/kg of body weight of FITC-labeled 3–5 kD molecule of dextran (Merck, Cat# FD4), and measured serum fluorescence 4 h later, as we published previously (35). TABL...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Publication date: Available online 16 July 2019Source: Journal of Advanced ResearchAuthor(s): Laura Botta, Luigino Dal Maso, Stefano Guzzinati, Chiara Panato, Gemma Gatta, Annalisa Trama, Massimo Rugge, Giovanna Tagliabue, Claudia Casella, Bianca Caruso, Maria Michiara, Stefano Ferretti, Flavio Sensi, Rosario Tumino, Federica Toffolutti, Antonio Giampiero Russo, Anna Luisa Caiazzo, Lucia Mangone, Walter Mazzucco, Silvia IacovacciAbstractThe aims of this study were to provide life expectancy (LE) estimates of cancer patients at diagnosis and LE changes over time since diagnosis to describe the impact of cancer during patien...
Source: Journal of Advanced Research - Category: Research Source Type: research
Publication date: Available online 17 July 2019Source: Academic RadiologyAuthor(s): Henkjan Huisman
Source: Academic Radiology - Category: Radiology Source Type: research
ConclusionIt is feasible to use CNN based algorithm to accurately distinguish cancerous regions in OCT images. This fully automated method can overcome limitations of manual interpretation including interobserver variability and speed of interpretation and may enable real-time intraoperative margin assessment.
Source: Academic Radiology - Category: Radiology Source Type: research
Authors: Farag AGA, Samaka R, Elshafey EN, Shehata WA, El Sherbiny EG, Hammam MA Abstract Background: Human JAKs are responsible for generating docking sites for human SSTAT phosphorylation. The role of JAKs in psoriasis pathogenesis has not been clearly explained. Aim: To investigate the role of JAK1 in psoriasis pathogenesis and to assess if this role is mediated through STAT3 or not, through evaluation of their immunohistochemical expression in the skin of psoriatic patients. Methods: This case-control study was carried out on 26 patients presenting with psoriasis vulgaris versus 26 age- and sex-matched apparent...
Source: Clinical, Cosmetic and Investigational Dermatology - Category: Dermatology Tags: Clin Cosmet Investig Dermatol Source Type: research
Conclusion: The findings showed Val92Met genotypes, sun exposure and family history were risk factors for melasma incidence. This is the first study on incidence of melasma in an Indonesian population and contributes to ongoing efforts to understand the mechanisms of melasma. PMID: 31308719 [PubMed]
Source: Clinical, Cosmetic and Investigational Dermatology - Category: Dermatology Tags: Clin Cosmet Investig Dermatol Source Type: research
Publication date: August 2019Source: Social Science &Medicine, Volume 235Author(s): Daiki Kobayashi, Rei Goto, Yusuke TsugawaAbstractEvidence is limited and mixed as to how improved price transparency affects patients' demand for healthcare. Price transparency usually affects both the supply and the demand side of healthcare. However, in Japan—where healthcare providers cannot compete on prices—we can examine an independent impact of price transparency on patients' demand for healthcare.The aim of this study is to investigate the impact of improved price transparency on patients' demand for healthcare. We c...
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
This study explores this question specifically in relation to cultural engagement (e.g. visiting museums/galleries/cinema/theatre/concerts) using data from 8,780 adults aged 50 + from the English Longitudinal Study of Ageing. We used a statistical triangulation approach, running three separate sets of analyses that each have different strengths and address different statistical limitations or biases. Using logistic regression, the relationship between cultural engagement and mental health was still present when including covariates relating to SES, and there was no evidence of moderation by SES either through the...
Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
Publication date: Available online 16 July 2019Source: Journal of Contextual Behavioral ScienceAuthor(s): Kitty Kioskli, Kirsty Winkley, Lance M. McCrackenAbstractPainful diabetic neuropathy (PDN) is a distressing and disabling condition. There is, surprisingly, relatively little research into the role of psychological variables related to PDN. The aim of this study was to investigate the association between psychological flexibility, daily functioning, and distress in people with PDN. This cross-sectional study included 225 participants who were recruited from hospital services and online. In correlation analyses, accepta...
Source: Journal of Contextual Behavioral Science - Category: Psychiatry & Psychology Source Type: research
We present a new database of Dutch word recognition times for a total of 54 thousand words, called the Dutch Crowdsourcing Project. The data were collected with an internet vocabulary test. The database is limited to native Dutch speakers. Participants were asked to indicate which words they knew. Their response times were registered, even though the participants were not asked to respond as fast as possible. Still, the response times correlate around .7 with the response times of the Dutch Lexicon Projects for shared words. Also results of virtual experiments indicate that the new response times are a valid addition to th...
Source: Psychologica Belgica - Category: Psychiatry & Psychology Source Type: research
In this study, a data processing pipeline for spatially resolved metabolomics analysis of tumor microregion heterogeneity was developed and built into a graphical interface with MSI software. Biological tissue sections were analysed by ambient air-flow assisted desorption electrospray ionization mass spectrometry imaging. Histology-driven and characterized ion images overlay combined with metabolic feature-based spatial segmentation were developed to accurately extract the metabolic profile from the tissue microregion of interest. In addition, appropriate data pretreatment methods were investigated to evaluate their abilit...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
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