Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic

In this study, the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau was compared to the BCG Pasteur reference strain. The methodology used was 2DE gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF), leading to the identification of 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits. Moonlighting proteins are multifunctional proteins in which two or more biological functions are fulfilled by a single polypeptide chain. Therefore, the identification of such proteins with moonlighting predicted functions can contribute to a better understanding of the molecular mechanisms unleashed by live BCG Moreau RDJ vaccine components. Introduction Tuberculosis (TB) is one of the 10 major causes of death worldwide. According to the World Health Organization (WHO), TB killed 1.7 million people in 2016 with 10.4 million new cases estimated worldwide ratifying the need for more effective treatment and prevention (1). To date, Bacillus Calmette-Guérin (BCG) is the only widely used prophylactic measure against TB (1). BCG is an attenuated Mycobacterium bovis strain obtained at the beginning of the Twentieth century at the Pasteur Institute, in Lille. It wa...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Publication date: February 2020Source: Infant Behavior and Development, Volume 58Author(s): Paulo Ricardo H. Rocha, Maria da C.P. Saraiva, Marco A. Barbieri, Alexandre A. Ferraro, Heloisa BettiolAbstractThe present study investigated the association between preterm birth PT conditions, intrauterine growth restriction IUGR and the combination of both PT-IUGR with infant motor development. A cohort with 1006 children was monitored during prenatal, at birth, and two years of age. Bayley-III screening was used to evaluate of fine and gross motor skills. The data did not indicate an increased risk for motor delays in the PT or ...
Source: Infant Behavior and Development - Category: Child Development Source Type: research
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Source: Journal of Food Composition and Analysis - Category: Food Science Source Type: research
Publication date: Available online 19 February 2020Source: International Journal of Gastronomy and Food ScienceAuthor(s): Máirtín Mac Con Iomaire
Source: International Journal of Gastronomy and Food Science - Category: Food Science Source Type: research
This study aimed to evaluate: (i) the effectiveness of plasma activated water (PAW) on the germination capacity of mung bean seeds and (ii) the antimicrobial capacity of PAW. A specially designed set-up composed of two electrochemical cell was used to produce two different types of PAW, by surface plasma discharge ignited from copper cathode and copper anode, respectively. The germination capacity of mung bean seeds was assessed over 96 h incubation in the dark at 25 °C. The antimicrobial capacity of PAW was assessed against 6 h exposure to Escherichia coli NCTC 12900. The anodic PAW (rich in NO3−) indicate...
Source: Food Control - Category: Food Science Source Type: research
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Source: Food Control - Category: Food Science Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
This article provides an overview of some of the major developments in this area over the past few years. In particular, it focuses on recent advances in the design and fabrication of multiple-bioactive delivery systems, plant-based delivery systems, and delivery systems for specific food applications.Graphical abstract
Source: Current Opinion in Food Science - Category: Food Science Source Type: research
The objective of this study was to characterize AKI in dogs with babesiosis caused by Babesia rossi at presentation and after treatment. Thirty-five client-owned dogs with B. rossi infection and 10 control dogs were included in this prospective observational study. Blood and urine were collected in Babesia-infected dogs at presentation (T0, n = 35), after 24 hours (T24h, n = 11), and after 1 month (T1m, n = 9). The following urinary kidney injury biomarkers were assessed: urinary protein to creatinine ratio (UPC), urinary glomerular injury biomarkers (immunoglobulin G (uIgG)...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
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Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
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