Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic

In this study, the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau was compared to the BCG Pasteur reference strain. The methodology used was 2DE gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF), leading to the identification of 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits. Moonlighting proteins are multifunctional proteins in which two or more biological functions are fulfilled by a single polypeptide chain. Therefore, the identification of such proteins with moonlighting predicted functions can contribute to a better understanding of the molecular mechanisms unleashed by live BCG Moreau RDJ vaccine components. Introduction Tuberculosis (TB) is one of the 10 major causes of death worldwide. According to the World Health Organization (WHO), TB killed 1.7 million people in 2016 with 10.4 million new cases estimated worldwide ratifying the need for more effective treatment and prevention (1). To date, Bacillus Calmette-Guérin (BCG) is the only widely used prophylactic measure against TB (1). BCG is an attenuated Mycobacterium bovis strain obtained at the beginning of the Twentieth century at the Pasteur Institute, in Lille. It wa...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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In this study, novel hollow gold nanoparticles (HGNPs) were used as drug carriers, and in order to improve the targeting ability of HGNPs to a lung tumor site, polyoxyethylene sorbitol oleate (PSO) was chosen here as a target ligand since it can be specifically recognized by the low-density lipoprotein (LDL) receptor which is usually over expressed on A549 lung cancer cells. In this way, a PSO-modified doxorubicin-loaded HGNP drug delivery system (PSO-HGNPs-DOX) was constructed and its physicochemical properties, photothermal conversion ability, and drug release of PSO-HGNPs-DOX was investigated. Further, the effects of tr...
Source: Drug Delivery - Category: Drugs & Pharmacology Tags: Drug Deliv Source Type: research
Authors: Hong D, Zhao HM Abstract Under the hydrothermal reaction condition, we prepared a new 3D Eu(III)-based coordination polymer (CP) [Eu(H2-DHBDC)0.5(DHBDC)0.5 (H2O)2]n·H2O (1, H4-DHBDC = 2,5-dihydroxy-1,4-terephthalic acid). Furthermore, we assessed the inhibitory influence on cervical cancer cells of this compound. Firstly, for testing the anti-viability of the compound against cervical cancer cells, the Cell Counting Kit-8 (CCK-8) assay was carried out. Then reactive oxygen species (ROS) detection kit was used to determine the ROS accumulation in the cervical cancer cells. Finally, after the Annexin ...
Source: Drug Development and Industrial Pharmacy - Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research
Authors: Fan S, Lin D, Wang Y Abstract PURPOSE: To evaluate the effects of prophylactic vitrectomy for rhegmatogenous retinal detachment (RRD) risk and visual outcome in acute retinal necrosis (ARN). METHODS: A systematic search of online databases was performed for articles published between 1994 June and 2020 March. The main outcomes that were assessed by a fixed effects model were RRD risk and visual outcome during a follow up period. RESULTS: A total of 7 trials involving 265 eyes (121 prophylactic vitrectomy eyes vs 144 routine antiviral treatment eyes) were analyzed in this study. RRD risk significant...
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
CONCLUSION: Targeted antiviral treatment and secondary recurrence prophylaxis prevent vision loss of the retina prior to immune recovery. PMID: 32966142 [PubMed - as supplied by publisher]
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
CONCLUSION: Severe extramedullary relapse in the eye might occur during chemotherapy intermission. PMID: 32965139 [PubMed - as supplied by publisher]
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
Authors: Yun J, Wu Z, Qi G, Han T, Zhang D Abstract INTRODUCTION: More attention has been paid to the eradication therapy of H. pylori with the enhancement of health awareness of patients. However, the increasing antibiotic resistance of H. pylori, due to the wide use and abuse of antibiotics, has become a critical factor affecting the efficacy of eradication. To effectively improve the eradication rate of H. pylori, high-dose amoxicillin-proton pump inhibitor (PPI) dual therapy has recently become one of the hot issues. AREAS COVERED: The authors review the schemes and efficacy of high-dose amoxicillin-PPI dua...
Source: Expert Review of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Expert Rev Gastroenterol Hepatol Source Type: research
Authors: Joussen AM, Müller B, Kirchhof B, Stappler T, Zeitz O Abstract Due to their complexity, globe ruptures are highly compromising traumas for the patient. This is due on the one hand to the eye injury itself with the accompanying loss of vision and on the other hand due to the need for extended treatment with uncertain prognosis and the resulting psychological stress. Globe ruptures are among the prognostically most unfavorable injuries due to the force and peak pressure impacting the eye. Furthermore, contusional retinal necrosis may be of significance prognostically. In the present review, we discuss t...
Source: Klinische Monatsblatter fur Augenheilkunde - Category: Opthalmology Tags: Klin Monbl Augenheilkd Source Type: research
CONCLUSION: SO remains a threatening clinical diagnosis that poses diagnostic and therapeutic challenges. It can be triggered post-traumatic, but also any intraocular surgery. This should be taken into account when assessing the indication for intraocular eye surgery, especially in eyes with reduced visual outcome. PMID: 32967030 [PubMed - as supplied by publisher]
Source: Klinische Monatsblatter fur Augenheilkunde - Category: Opthalmology Tags: Klin Monbl Augenheilkd Source Type: research
Authors: Tost F, Stahl A Abstract The ophthalmologic assessment of causal relationships is subject to formal guidelines, depending on the legal field (social law in the statutory accident insurance, civil law in the private accident insurance). After determining all objective and subjective findings of the individual case with complete recording of the medical facts, the ophthalmologist has the task of making a summarizing assessment of the existing cause-and-effect relationship. With regard to the distinction between retinal damage caused by an accident or retinal disease not caused by an accident, it is necessary...
Source: Klinische Monatsblatter fur Augenheilkunde - Category: Opthalmology Tags: Klin Monbl Augenheilkd Source Type: research
Publication date: Available online 23 September 2020Source: The Annals of Thoracic SurgeryAuthor(s): Kiyomichi Mizuno, Mitsuhiro Isaka, Mototsugu Ono, Takamitsu Hayakawa, Yukihiro Terada, Yoshiyuki Yasuura, Hiroyuki Kayata, Hayato Konno, Hideaki Kojima, Tetsuya Mizuno, Takanori Kawabata, Yasuhisa Ohde
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
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