Dipotassium Glycyrrhizate Improves Intestinal Mucosal Healing by Modulating Extracellular Matrix Remodeling Genes and Restoring Epithelial Barrier Functions

In conclusion, this study shows for the first time that: (1) DPG is able to modulate in vivo MH genes and restore in vitro the proper structural organization, wound repair ability and functionality of intestinal epithelial barrier during acute inflammation; (2) DPG increases ECM remodeling gene expression during the earlier recovery phase following the induction of colitis in mice; (3) the ECM genes, PLAUR and VTN, are pivotal to get MH in cells treated with DPG. We are suggesting that the use of DPG would represent in the future an innovative and powerful tool for the treatment of human inflammatory complex disorders, including IBD. Ethics Statement Experimental procedures were previously approved by the Ministry of Health and the study was carried out in accordance with the Italian regulations on animal welfare. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Italian National Agency for New Technology, Energy and Sustainable Economic Development (ENEA-Permit Number: 1175/2016-PR). Author Contributions LS and RV conceived and designed experiments. LS, FP, and ABM performed experiments and analyzed data. AN contributed to data analysis. EC and VC supervised in vitro analysis. SC and SI supervised the work. LS and RV wrote the manuscript. Funding This work was supported by Accademic Funding of Sapienza University of Rome. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any comme...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research