Human Double-Negative Regulatory T-Cells Induce a Metabolic and Functional Switch in Effector T-Cells by Suppressing mTOR Activity
In conclusion, our results reveal new and various targets of DN T-cells to selectively modulate signaling and metabolic programming of T-cells resulting in functional altered effector cells. These findings could pave the way to use DN T-cells for cellular therapy as an alternative treatment strategy to prevent and diminish GvHD after allo-HSCT.
Ethics Statement
This study was carried out in accordance with the recommendations of Ethics committee of the University Erlangen-Nuremberg with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Ethics committee of the University Erlangen-Nuremberg (protocol number 284-18 Bc).
Author Contributions
TH, AM, and SV designed the research. TH, MA, MP, CS, and SV performed experiments. TH, MA, KH, DM, HB, AM, and SV analyzed and interpreted data. TH, AM, and SV wrote the manuscript. HB, DM, and AM provided advice and revised the manuscript. All of the authors edited the manuscript.
Funding
This work was supported by the doctoral training program GRK1660, by the CRC1181 (project B04), and by the TRR221 (project no. 324392634) from the German Research Foundation (DFG).
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank Dorothea Gebhardt and Ja...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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