In silico Selection of Amplification Targets for Rapid Polymorphism Screening in Ebola Virus Outbreaks

Discussion Genome comparison of over 1,230 non-redundant, high quality EBOV full-length sequences within the Zaire lineage revealed both conserved and highly variable regions (Figure 1). The latter were concentrated in non-coding sequences, which were also more AT-rich than coding sequences, an observation that has also been made for other virus species, for instance Hepatitis B virus (González et al., 2018). The AT-content of coding sequences was most likely lower due to codon constraints, though we observe that the gene for RNA polymerase is richer in AT than the other EBOV genes. The analysis further identified the presence of local inverted repeats in intergenic regions that are likely to function in translation termination. The intergenic region between NP and VP35 was rich in AT but not enriched in polymorphic sequences, which may indicate other local constraints for conservation. One possibility is the presence of a pre-microRNA, whose processing would depend on conservation of inverted repeats (Liu et al., 2016). Indeed, these are present in the intergenic region of NP and VP35, although, to the best of our knowledge, no miRNA has yet been identified for this location. Commercial companies producing drugs and detection technology are mostly interested in EBOV genomic regions that are highly conserved, as their products should not be affected by ongoing genetic changes. The three extended highly conserved regions identified, in genes coding for NP, VP40 and VP...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

This article analyzes the burden of COVID-19 on patients with opioid addiction and discusses the beneficial policy changes that improve access to substance abuse care.
Source: Journal of the American Academy of Physician Assistants - Category: Primary Care Tags: Special Article Source Type: research
The number of people living with chronic immunosuppression is increasing in the United States. Patients with HIV, those who have had bone marrow or solid organ transplants, and patients taking biologics for autoimmune diseases are at increased risk for skin cancer. Skin cancer in these patients is more aggressive and more likely to metastasize and cause death. Medications and individual risk factors such as sex, age, and ethnicity are independent risk factors for the development of skin cancer. Routine screening and aggressive treatment of actinic keratoses and nonmelanoma skin cancers can reduce patients' skin cancer burd...
Source: Journal of the American Academy of Physician Assistants - Category: Primary Care Tags: CME: Primary Care Source Type: research
This article describes a patient who presented to the ED with jaundice and acute liver injury, which after a thorough exclusion of alternative causes was attributed to kratom use.
Source: Journal of the American Academy of Physician Assistants - Category: Primary Care Tags: Case Report Source Type: research
Thursday is the deadline for health care workers to get at least their first dose of coronavirus vaccine in much of the country, as the Biden administration’s mandate starts to take effect.
Source: Washington Post: To Your Health - Category: Consumer Health News Authors: Source Type: news
The CDC contacted pharmacies on Wednesday to reinforce the message that people with moderate to severe immune suppression should receive a fourth COVID-19 vaccine.WebMD Health News
Source: Medscape Infectious Diseases Headlines - Category: Infectious Diseases Tags: Infectious Diseases News Source Type: news
GAO criticizes the response to emergencies over four administrations, including coronavirus, Ebola and Zika, as well as extreme weather events.
Source: Washington Post: To Your Health - Category: Consumer Health News Authors: Source Type: news
This study aimed to present data on healthcare worker (HCW) screening, status of HCW, pre-interventional testing, the use of personal protective equipment (PPE) and the economic impact of the pandemic.MethodsOtolaryngological private practices and hospital-based departments were surveyed nationwide using an online questionnaire. Participating facilities were recruited via the German Society for Oto-Rhino-Laryngology and the German Association for Otolaryngologists in Bavaria.Results365 private practices (2776 employees) and 65 hospitals (2333 employees) were included. Significantly more hospitals (68.7%) than practices (40...
Source: European Archives of Oto-Rhino-Laryngology - Category: ENT & OMF Source Type: research
AbstractPurposeEvaluating the current health state in chronic otitis media (COM), audiologic results are complemented by subjective outcomes, such as health-related quality of life (HRQoL). Two disease-specific instruments assessing HRQoL in COM in German-speaking patients exist, i.e., the chronic otitis media outcome test (COMOT-15) and the Zurich chronic middle ear inventory (ZCMEI-21). Since the psychometric properties of these questionnaires in a concurrent application are unknown, the aim of this study was to compare the COMOT-15 and the ZCMEI-21.MethodsHRQoL was assessed in adult COM patients using the COMOT-15 and t...
Source: European Archives of Oto-Rhino-Laryngology - Category: ENT & OMF Source Type: research
The U.S. Army is working on a pan-coronavirus vaccine that will be effective against all future strains of the virus, and could even prevent future pandemics from occuring.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
The last recorded sighting of a leafhopper from the same genus as Phlogis kibalensis was in 1969A new species of insect has been found in the Ugandan rainforest that belongs to a group of insects so rare that its closest known relative was last seen more than 50 years ago.The species of leafhopper, namedPhlogis kibalensis, was discovered by a British scientist doing field work in a national park in western Uganda.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Insects Science Uganda Wildlife UK news World news Anglia Ruskin University Animals Africa Environment National parks Source Type: news
More News: Academia | African Health | Congo Health | Databases & Libraries | Ebola | Ebola Vaccine | Endemics | Environmental Health | Filovirus | Funding | Gastroenterology | Gene Therapy | Genetics | Germany Health | Greece Health | Guinea Health | Hepatitis | Hepatitis B | Hepatitis Vaccine | History of Medicine | International Medicine & Public Health | Lessons | Merck | Microbiology | Mumps | Mumps Vaccine | Outbreaks | Oxford University | Reproduction Medicine | Study | Sudan Health | Thailand Health | Uganda Health | UK Health | USA Health | Vaccines | Virology | WHO | Zika Virus