In silico Selection of Amplification Targets for Rapid Polymorphism Screening in Ebola Virus Outbreaks

Discussion Genome comparison of over 1,230 non-redundant, high quality EBOV full-length sequences within the Zaire lineage revealed both conserved and highly variable regions (Figure 1). The latter were concentrated in non-coding sequences, which were also more AT-rich than coding sequences, an observation that has also been made for other virus species, for instance Hepatitis B virus (González et al., 2018). The AT-content of coding sequences was most likely lower due to codon constraints, though we observe that the gene for RNA polymerase is richer in AT than the other EBOV genes. The analysis further identified the presence of local inverted repeats in intergenic regions that are likely to function in translation termination. The intergenic region between NP and VP35 was rich in AT but not enriched in polymorphic sequences, which may indicate other local constraints for conservation. One possibility is the presence of a pre-microRNA, whose processing would depend on conservation of inverted repeats (Liu et al., 2016). Indeed, these are present in the intergenic region of NP and VP35, although, to the best of our knowledge, no miRNA has yet been identified for this location. Commercial companies producing drugs and detection technology are mostly interested in EBOV genomic regions that are highly conserved, as their products should not be affected by ongoing genetic changes. The three extended highly conserved regions identified, in genes coding for NP, VP40 and VP...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Abstract The outbreak of Coronavirus Disease 2019 (COVID-19) caused a worldwide pandemic. Less than 6 weeks after the first confirmed cases in Korea, the patient number exceeded 5,000, which overcrowded limited hospital resources and forced confirmed patients to stay at home. To allocate medical resources efficiently, Korea implemented a novel institution for the purpose of treating patients with cohort isolation out of hospital, namely the Community Treatment Center (CTC). Herein, we report results of the initial management of patients at one of the largest CTC in Korea. A total of 309 patients were admitted to o...
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Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
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Source: SafetyLit - Category: International Medicine & Public Health Tags: Jurisprudence, Laws, Legislation, Policies, Rules Source Type: news
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Source: SafetyLit - Category: International Medicine & Public Health Tags: Alcohol and Other Drugs Source Type: news
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