C-type lectin response to bacterial infection and ammonia nitrogen stress in tiger shrimp (Penaeus monodon)

Publication date: Available online 24 April 2019Source: Fish & Shellfish ImmunologyAuthor(s): Yukai Qin, Shigui Jiang, Jianhua Huang, Falin Zhou, Qibin Yang, Song Jiang, Lishi YangAbstractC-type lectins (CTLs) are pattern recognition receptors (PRRs) that are important in invertebrate innate immunity for the recognition and elimination of pathogens. A new type of CTL was characterized from tiger shrimp (Penaeus monodon) and designated as PmCL1. The full-length cDNA of PmCL1 was 861 bp long, with a 495-bp open reading frame, encoding a 164-amino acid protein. The mRNA transcript of PmCL1 showed the highest expression in the hepatopancreas, whereas it was barely detected in the ovary. After the shrimp were stimulated by Vibrio harveyi and Vibrio anguillarum, PmCL1 expression in the hepatopancreas and gill was significantly upregulated. A carbohydrate-binding assay revealed the specificity of PmCL1 for pathogen-associated molecular patterns (PAMPs) that included peptidoglycan (PGN) and lipopolysaccharide (LPS), and saccharides that included d-glucose, galactosamine, α-lactose, treholose, and d-mannose. Recombinant PmCL1 agglutinated gram-positive (Staphylococcus aureus) and gram-negative bacteria (V. harveyi, V. anguillarum, Vibrio alginolyticus, Vibrio parahemolyticus, Vibrio vulnificus, and Aeromonas hydrophila) in the presence of calcium ions and enhanced the efficiency of clearing the invading bacteria. Collectively, our results suggested that PmCL1 might play an important ...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research