Cobalt-chromium-enriched medium ameliorates shear-stressed endothelial cell performance

Publication date: Available online 24 April 2019Source: Journal of Trace Elements in Medicine and BiologyAuthor(s): Mariana Issler Pinheiro Machado, Anderson Moreira Gomes, Marcel Ferreira Rodrigues, Thais Silva Pinto, Célio Júnior da Costa Fernandes, Fábio J. Bezerra, Willian Fernando ZambuzziABSTRACTAngiogenesis is a relevant mechanism to be considered for the success of bone healing, even considering endosseous implantable devices, providing adequate delivery of substances necessaries for the cell viability and bone de novo deposition. Within of the repertory of metal-based implantable alloys, cobalt-chromium (CoCr) has emerged with very interesting properties for biomedical applications. Additionally, we have shown that released molecules from implants devices are able to modulate cells away and because that we hypothesized these released molecules might act on endothelial cells. In order to better address this issue, we investigated the effect of Co-Cr-enriched medium on endothelial cells (HUVECs), considering a biological model subjecting those cells to shear-stress to partially mimic the physiological environment and further allow investigating intracellular pathways responsible to drive cytoskeletal rearrangement, cell viability and extracellular matrix (ECM) remodeling processes. Considering the analysis of the metalloproteinases (MMPs) activities, our data indicates an intense ECM remodeling in response to CoCr-enriched medium suggesting some role on angiogenesis...
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research