Systems Biology Analysis of the Effect and Mechanism of Qi-Jing-Sheng-Bai Granule on Leucopenia in Mice

In this study, we firstly tested the effects of QJSB on leucopenia using mice induced by cyclophosphamide. Our results suggested that QJSB significantly raised the number of peripheral white blood cells, platelets and nucleated bone marrow cells. Additionally, it markedly enhanced the cell viability and promoted the colony formation of bone marrow mononuclear cells. Furthermore, it reversed the serum cytokines IL-6 and G-CSF disorders. Then, using transcriptomics datasets and metabonomic datasets, we integrated transcriptomics-based network pharmacology and metabolomics technologies to investigate the mechanism of action of QJSB. We found that QJSB regulated a series of biological processes such as hematopoietic cell lineage, homeostasis of number of cells, lymphocyte differentiation, metabolic processes (including lipid, amino acid, and nucleotide metabolism), B cell receptor signaling pathway, T cell activation and NOD-like receptor signaling pathway. In a summary, QJSB has protective effects to leucopenia in mice probably through accelerating cell proliferation and differentiation, regulating metabolism response pathways and modulating immunologic function at a system level. Introduction Chemotherapy has been widely used for the treatment of cancers. However, chemotherapy usually induces many adverse effects, including hematologic toxicity and neurotoxicity (Magge and DeAngelis, 2015; Ratti and Tomasello, 2015; Zhou et al., 2018). Among them, hematologic toxicity, ...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research

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This article presents a unique 3D biocompatible Aluminum-based quantum structure (QS) for in vitro cancer detection using Surface Enhanced Raman Scattering (SERS). The Al-based QSs fabricated using ultrashort pulsed laser are of two distinct surface characters, wrinkled and smooth spherical. The limit of detection for chemical sensing of Crystal Violet and Rhodamine 6G by the Al-QS was driven up to single molecule sensing (femtomolar concentration). Biological sensing of cysteine, a disease biomarker and carcinoembryonic antigen (CEA), a cancer biomarker was also tested by the Al-QS. The ability of in vitro cell detection ...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research
In this study, four different weight% of Punica granatum fruit peel extract were used as green stabilizers to synthesize IONPs followed by a series of characterization experiments and in vitro anticancer evaluation. Results revealed that all synthesized IONPs have high degree of crystallinity and purity. The optimum saturation magnetization, and hydrodynamic size were found to be ∼69 emu.g−1, and 26.52 nm, respectively. Morphological studies demonstrated that adding peel extract decreased size of NPs with the average particle size below 11 nm. Cytotoxicity assay showed that the IONPs were not react...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: 15 March 2020Source: Journal of Molecular Structure, Volume 1204Author(s): A.D. Herrera-España, J. Us-Martín, S. Hernández-Ortega, G. Mirón-López, L. Quijano, J.R. Villanueva-Toledo, G.J. Mena-RejónAbstractThe structural modification of natural products has shown to be a remarkable tool in the generation of novel bioactive molecules. Herein, the synthesis, structural analysis and anticancer activity of the novel triterpenoid thiazole 3 is reported. The synthesis was carried out through the bromination of ochraceolide A (1) and the subsequent heterocyclic condensat...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: Available online 15 December 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Shengming Sun, Ying Wu, Ivan Jakovlić, Hongtuo Fu, Xianping Ge, Hui Qiao, Wenyi Zhang, Shubo JinAbstractThe oriental river prawn, Macrobrachium nipponense, is a commercial freshwater prawn species in China. It is highly sensitive to hypoxia, and this has posed a challenge to its intensive culturing. To date, the effects of hypoxia on reproduction in female prawns are not entirely clear, as are the underlying mechanisms of the effects of hypoxia. In this work, comparative t...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Category: Molecular Biology Source Type: research
Publication date: Available online 15 December 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): ChenChen Bian, Xiaocheng Huang, Zechao Hu, Shanghong Ji, Minghui Yang, Hong JiAbstractGlycogen synthase kinase-3β (GSK-3β) plays a crucial role in regulating lipid metabolism, endoplasmic reticulum (ER) stress and apoptosis in mammals, however, its gene structure and function is little known about in fish. Here, its two paralogs, grass carp (Ctenopharyngodon idella) GSK-3β1 and GSK-3β2 were isolated and characterized, encoding peptides of 421 and 457 ami...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Category: Molecular Biology Source Type: research
In this study, European eels (Anguilla anguilla) were immunized by the bivalent expression products of the outer membrane protein (Omp) gene from A. hydrophila (OmpⅡ) and E. anguillarum (OmpA), and the effects of the bivalent protein (rOmpⅡ-A) on the immune function of the European eel were detected. Three hundred eels were divided average into three groups of PBS, adjuvant and rOmp. Eels of three goups were injected intraperitoneal with 0.2 mL of PBS (0.01 mol/L, pH7.4), PBS + F (PBS mixed equal volume of freund's uncomplete adjuvant) or rOmpⅡ-A (1 mg mL−1 rOmpⅡ-A mixed equal vo...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In this study, we demonstrated that proteasome subunit beta-type 7 (PSMB7), a proteasome-constitutive protein that is important for proteasome assembly, interacts with grass carp reovirus (GCRV) capsid proteins. Yeast 2-hybrid assay indicates that capsid protein VP38 of genotype Ⅲ GCRV could bind PSMB7, and this mutual interaction was further confirmed by pull-down, co-immunoprecipitation and subcellular co-localization assays. Furthermore, VP38 homologous proteins, VP7 from genotype I and VP35 from genotype II GCRV, can also interact with host PSMB7 in similar protein-protein interaction assays. Finally, PSMB7 expressio...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In this study, two groups (4% PHB supplementation diets and control with basal diets) with 30-day feeding were set to study the effect of PHB supplementation on immune-related gene expression by qRT-PCR, time-course CyHV-2 replication in vivo by qPCR and intestinal microbiota by illumine high-throughput sequencing. PHB supplementation significantly up-regulated transcriptional levels of eight immune-related genes, decreased cumulative mortality of gibel carp and early CyHV-2 replication in spleen in vivo (P 
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 14 December 2019Source: Fish &Shellfish ImmunologyAuthor(s): Xueqing Han, Fengying Gao, Maixin Lu, Zhigang Liu, Miao Wang, Xiaoli Ke, Mengmeng Yi, Jianmeng CaoAbstractInterleukin-1 receptor-associated kinase 1 (IRAK1) and IRAK4 are critical signalling mediators and play pivotal roles in the innate immune and inflammatory responses mediated by TLR/IL-1R. In the present study, two IRAK family members, OnIRAK1 and OnIRAK4, were identified in the Nile tilapia Oreochromis niloticus with a conserved N-terminal death domain and a protein kinase domain, similar to those of other fishes and ma...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
ConclusionInfliximab was not efficient in reducing depressive symptoms according to the HAM-D, only when the patients already had increased inflammatory genes, including TNF and C-reactive protein (CRP).
Source: Pharmacology Biochemistry and Behavior - Category: Biochemistry Source Type: research
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