Subcutaneous Adipose Tissue Free Fatty Acid Uptake Measured Using Positron Emission Tomography and Adipose Biopsies in Humans.

Subcutaneous Adipose Tissue Free Fatty Acid Uptake Measured Using Positron Emission Tomography and Adipose Biopsies in Humans. Am J Physiol Endocrinol Metab. 2019 Apr 23;: Authors: Cao Y, Gathaiya N, Han Q, Kemp BJ, Jensen MD Abstract PET radiopharmaceuticals can noninvasively measure free fatty acid (FFA) uptake into adipose tissue. We studied 29 volunteers to test whether abdominal and femoral subcutaneous adipose tissue FFA uptake measured using [1-11C]palmitate PET agrees with FFA storage rates measured using an intravenous bolus of [1-14C]palmitate and adipose biopsies. The dynamic left ventricular cavity PET images combined with blood sample radioactivity corrected for the 11CO2 content were used to create the blood time activity curve (TAC) and the constant (Ki) was determined using Patlak analysis of the TACs generated for regions of interest in abdominal subcutaneous fat. This data was used to calculate palmitate uptake rates in abdominal subcutaneous adipose tissue (µmol•kg-1•min-1). Immediately after the dynamic imaging a static image of the thigh was taken to measure standardized uptake values (SUV) in thigh adipose tissue, which was scaled to each participant's abdominal adipose tissue SUV to calculate thigh adipose palmitate uptake rates. Abdominal adipose palmitate uptake using PET [1-11C]palmitate was correlated with, but significantly (p < 0.001) greater than FFA storage measure using [1-14C]palmitate and adi...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research