Prognostic and Predictive Value of Three DNA Methylation Signatures in Lung Adenocarcinoma

In this study, based on the whole-genome methylation datasets from The Cancer Genome Atlas (TCGA) and several machine learning methods, we evaluated the possibility of DNA methylation signatures for identifying lymph node metastasis of LUAD, differentiating between tumor tissue and normal tissue, and predicting the overall survival (OS) of LUAD patients. Using the regularized logistic regression, we built a classifier based on the 3616 CpG sites to identify the lymph node metastasis of LUAD. Furthermore, a classifier based on 14 CpG sites was established to differentiate between tumor and normal tissues. Using the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression, we built a 16-CpG-based model to predict the OS of LUAD patients. Results: With the aid of 3616-CpG-based classifier, we were able to identify the lymph node metastatic status of patients directly by the methylation signature from the primary tumor tissues. The 14-CpG-based classifier could differentiate between tumor and normal tissues. The area under the receiver operating characteristic (ROC) curve (AUC) for both classifiers achieved values close to 1, demonstrating the robust classifier effect. The 16-CpG-based model showed independent prognostic value in LUAD patients. Interpretation: These findings will not only facilitate future treatment decisions based on the DNA methylation signatures but also enable additional investigations into the utilization of LUAD DNA methylation pattern by di...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research

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Source: Immunological Investigations - Category: Allergy & Immunology Tags: Immunol Invest Source Type: research
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Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
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Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
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Source: virology blog - Category: Virology Authors: Tags: This Week in Virology ACE2 China coronavirus CoV COVID-19 epidemic evolution furin pangolin pneumonia receptor binding domain respiratory aerosol SARS-CoV-2 spike glycoprotein viral viruses Wuhan zoonosis Source Type: blogs
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