Insights Into Dolphins' Immunology: Immuno-Phenotypic Study on Mediterranean and Atlantic Stranded Cetaceans

This study was aimed at implementing the knowledge on the immune response in cetaceans stranded along the Italian coastline to provide a baseline useful for assessing the immune status of bottlenose (Tursiops truncatus) and striped (Stenella coeruleoalba) dolphins. In particular, since the Mediterranean Sea is considered a heavily polluted basin, a comparison with animals living in open waters such as the Atlantic Ocean was made. Formalin-fixed, paraffin-embedded spleen, thymus, and lymph node tissues from 16 animals stranded along Italian and 11 cetaceans from the Canary Island shores were sampled within 48 h from death. Information regarding stranding sites, gender, and age as well as virologic, microbiological, and parasitological investigations, and the cause and/or the death mechanism were also collected in order to carry out statistical analyses. Selected tissues were routinely stained with hematoxylin-eosin (H&E) and with immunohistochemical techniques (IHC). For IHC analysis, anti-human CD5 monoclonal mouse antibody to identify T lymphocytes, CD20 monoclonal mouse antibody for the identification of mature B lymphocytes and HLA-DR antigen (alpha-chain) monoclonal mouse antibody for the identification of the major histocompatibility complex type II were previously validated for both species by Western-blotting technique. T-test method applied to quantitative evaluation of IHC positive cells showed a significant relationship between the number of (expression) of CD20 sta...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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In this study, 5% w/v SMP dispersion was acidified to pH 2.4–3.0 using citric acid and heated at different conditions. Lowering pH resulted in dissolution of colloidal calcium phosphate from casein micelles, corresponding to lowered turbidity and reduced hydrodynamic diameter of dispersions. When heated for 10 min at different temperatures, 70 °C was the most effective to produce transparent dispersions; whereas a shorter heating time between 2 and 60 min was desired at 90 °C. The decreased turbidity after heating was attributed to weakened hydrophobic interaction between casein molecules based on f...
Source: Food Hydrocolloids - Category: Food Science Source Type: research
In this study, we developed a methodology combined Paternò-Büchi reaction nanoelectrospray ionization mass spectrometry (PB-nanoESI-MS) and nuclear magnetic resonance (NMR) to identify C=C locations and isomers of PUTAGs in sacha inchi oil. Benzophenone was used as the PB reagent, and the optimized solvent composition (methanol:chloroform=9:1) allowed for PUTAGs and their PB products to be detected with higher intensities. In addition, we made efforts to interpret the MS2 spectra for identification lipid species. A series of C57-PUTAGs and C59-PUTAGs were detected and identified via high-resolution PB-nanoESI-M...
Source: Food Chemistry - Category: Food Science Source Type: research
This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The cheeses made using CpCP3 showed higher moisture content than those made with chymosin, but protein, fat, and ash were similar. The sensory analysis showed that cheeses made with CpCP3 had high acceptance index ...
Source: Food Chemistry - Category: Food Science Source Type: research
We report a molecular dynamics investigation on dilute and concentrated solutions of four acidic organophosphoric extractants (P507, P204, P227 and Cyanex272) in pure oil phase and oil-water biphasic systems for purpose of depicting solution and interface behaviors, which has been a cornerstone of chemistry in solvent extraction and separation. We can intuitively find that the single extractant promotes the interface movement. When the extractants get concentrated in organic phase, they diffuse and aggregate to dimers and trimers. In oil-water biphasic system, most of the extractants tend to migrate to the interface and re...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 13 October 2019Source: Journal of Molecular LiquidsAuthor(s): Hany M.Abd El-Lateef, Zeinab A. Abdallah, Mohamed S.Mohamed AhmedAbstractEthyl 2-(1,2,3,6-tetrahydro-6-oxo-2-thioxopyrimidin-4-yl)ethanoate (EOTPE) was synthesized by a facile and cost-effective method. The structure confirmation of the synthesized EOTPE was performed by different spectroscopic methods. The corrosion inhibition action of EOTPE in pickling acids (1.0 N HCl and1.0 N H2SO4) at different temperatures (30–60 °C) was tested by various surface and corrosion methods, including open circuit potential (...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Org. Biomol. Chem., 2019, Accepted Manuscript DOI: 10.1039/C9OB02014D, CommunicationBing-Ke Ning, Hao-Jie Rong, Cui-Feng Yang, Tao Chen, Ze-Gang Xu, Tian-Duo Su, Yong-Qiang Wang Herein we report that iodine-catalyzed guanylation of primary amines can be accomplished with N,N ′-di-Boc-thiourea and TBHP to afford the corresponding guanidines in 40–99% yields. Oxidation of the HI byproduct by... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Organic and Biomolecular Chemistry - Category: Molecular Biology Authors: Source Type: research
Publication date: November 2019Source: Fish &Shellfish Immunology, Volume 94Author(s): Elumalai Preetham, Abdul Salam Rubeena, Baskaralingam Vaseeharan, Mukesh Kumar Chaurasia, Jesu Arockiaraj, Rolf Erik OlsenAbstractThe study is carried out to understand the antimicrobial and immunological response of a potential immune molecule lectin, MmLec isolated from haemolymph of Speckled shrimp, Metapenaeus monoceros. MmLec was purified using mannose coupled Sepharose CL-4B affinity chromatography, which was further subjected on SDS-PAGE to ascertain the distribution of their molecular weight. Sugar binding specificity assay w...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 13 October 2019Source: Carbohydrate PolymersAuthor(s): Shaolei Kang, Hualin Wang, Li Xia, Minmin Chen, Linlin Li, Junfeng Cheng, Xingjiang Li, Shaotong JiangAbstractIn this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film. The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid. The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA. An appropriate content of RAs ...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 13 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Zakie Anajafi, Mahmoud Naseri, G. NeriAbstractIn this paper, the synthesis, characterization and sensing properties of SmFeO3/ZnO nanocomposite synthesized by the thermal treatment method, is reported. The morphological and microstructural characteristics of the synthesized composite nanopowders and single SmFeO3 and ZnO components, were analyzed by Scanning Electron Microscopy-Electron Dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), Fourier Transform-Infrared (FT-IR) spectroscopy and Photoluminscence (PL). Characteri...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Publication date: Available online 13 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Chenyu Yao, Limin Xiao, Shoufei Gao, Yingying Wang, Pu Wang, Ruifeng Kan, Wei Jin, Wei RenAbstractWe demonstrated the sensitive CO detection in a novel hollow-core negative curvature fiber (HC-NCF) with infrared laser absorption at 2.3 µm. The HC-NCF consists of a single ring of eight nontouching silica capillaries around the air core, providing a single-mode light delivery of the 2.3-µm distributed feedback laser. A high coupling efficiency of 90% was achieved with the aid of optimal free-space coupling...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
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