Propofol promotes apoptosis of colorectal cancer cells via alleviating the suppression of lncRNA HOXA11-AS on miRNA let-7i.

Propofol promotes apoptosis of colorectal cancer cells via alleviating the suppression of lncRNA HOXA11-AS on miRNA let-7i. Biochem Cell Biol. 2019 Apr 23;: Authors: Ren YL, Zhang W Abstract Recently, surgical resection is still the mainstay for colorectal cancer (CRC) treatment. Propofol (2,6-diisopropylphenol), one of the most commonly used intravenous anaesthetic agents, has been reported to be involved in modulating the malignancy of a variety of human cancers. However, the underlying mechanisms remain poorly understood. In the present study, we found that propofol could promote cell apoptosis and inhibit cell proliferation using Cell Counting Kit-8 (CCK-8), flow cytometry and caspase 3 cleavage assays, which causing the down-regulation of HOXA11-AS and up-regulation of let-7i in both Colo205 and SW620 cells. Moreover, gain-of-function studies of HOXA11-AS or loss-of-function studies of let-7i also revealed that a negative correlation between HOXA11-AS and let-7i in propofol-mediated biological functions of colorectal cancer cells. Furthermore, the mechanistic experiments revealed that HOXA11-AS acts as a molecular sponge for let-7i, which to regulate ABCC10 expression. Taken together, we investigate that propofol suppresses colorectal cancer tumorigenesis by modulating HOXA11-AS/let-7i/ABCC10 regulatory network, indicating a potential mechanism of propofol in CRC development. PMID: 31013434 [PubMed - as supplied by publi...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research