Physical Extraction and Fast Protein Liquid Chromatography for Purifying Flagella Filament From Uropathogenic Escherichia coli for Immune Assay

In this study, we sought to establish a new, carefully optimized method to extract and purify non-denatured, native FliC from the reference UPEC strain CFT073 to be suitable for immune assays. To achieve purification of FliC to homogeneity, we used a mutant CFT073 strain containing deletions in four major chaperone-usher fimbriae operons (type 1, F1C and two P fimbrial gene clusters; CFT073Δ4). A sequential flagella extraction method based on mechanical shearing, ultracentrifugation, size exclusion chromatography, protein concentration and endotoxin removal was applied to CFT073Δ4. Protein purity and integrity was assessed using SDS-PAGE, Western blots with anti-flagellin antisera, and native-PAGE. We also generated a fliC-deficient strain, CFT073Δ4ΔfliC, to enable the concurrent preparation of a suitable carrier control to be applied in downstream assays. Innate immune stimulation was examined by exposing J774A.1 macrophages to 0.05-1 μg of purified FliC for 5 h; the supernatants were analyzed for cytokines known to be induced by flagella, including TNF-α, IL-6, and IL-12; the results were assessed in the context of prior literature. Macrophage responses to purified FliC encompassed significant levels of several cytokines consistent with prior literature reports. The purification method described here establishes a new approach to examine highly purified FliC in the context of host-pathogen interaction model systems. Introduction Flag...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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This study aimed to evaluate the success rate and image quality of silent MRI for the brain of children.Materials and MethodsThis was a retrospective study of 3–36-month children who underwent silent or conventional brain MRI at the People's Hospital of Northern Jiangsu from 01/2015 to 02/2018. The success rates were compared. The acoustic noise of each sequence was measured using a decibel meter. The signal-to-noise ratio and contrast-to-noise ratio of the diffusion-weighted imaging, T2W, and T1W sequences were analyzed. Subjective image quality (lesion delineation, visibility, gray-white differentiation, and overal...
Source: Academic Radiology - Category: Radiology Source Type: research
Publication date: 1 January 2020Source: Personality and Individual Differences, Volume 152Author(s): Philip J. CorrAbstractThis Special Issue poses a pertinent question: Is a consensual paradigm needed, possible, or even desirable, in personality psychology? One seems necessary to unify the disparate perspectives that characterise the field, as well as to make a major contribution to the broader unification of psychology in which individual differences loom large. This discussion is presented in relation to standard models in mature science where scientific progress is more assured. Additionally, such a consensual paradigm...
Source: Personality and Individual Differences - Category: Psychiatry & Psychology Source Type: research
Authors: Gillis LJ Abstract Obesity is a major health issue. A community-based intervention was implemented on a college campus to help employees lose body weight and fat. Participants were scanned using the InBody device at pre- and post-intervention, measuring body weight, fat, and waist circumference. Participants attended 11 classes on healthy lifestyle education. A comparison of pre- and post-intervention measurements was made using a paired t-test, resulting in a statistically significant difference in outcome measures. Correlation between class attendance and pounds lost showed a moderate negative correlatio...
Source: Journal of Community Health Nursing - Category: Nursing Tags: J Community Health Nurs Source Type: research
Publication date: Available online 17 October 2019Source: Pathology - Research and PracticeAuthor(s): Jia Yang, Junjie Shangguan, Aydin Eresen, Yu Lia, Jian Wang, Zhuoli ZhangAbstractDespite significant advances over the past decades of research, pancreatic cancer (PC) continues to have the worst 5-year survival of any malignancy. Dendritic cells (DCs) are the most potent professional antigen-presenting cells and are involved in the induction and regulation of antitumor immune responses. DC-based immunotherapy has been used in clinical trials for PC. Although safety, efficacy, and immune activation were reported in patient...
Source: Pathology Research and Practice - Category: Pathology Source Type: research
ConclusionsThe efficacy of crizotinib in patients with advanced NSCLC is related to the number of metastatic organs, age and timing of treatment. The use of crizotinib is prone to intracranial progression, and progression of simple brain metastases is not an indication that crizotinib is discontinued. Patients will continue to benefit from combination of local radiotherapy.
Source: Pathology Research and Practice - Category: Pathology Source Type: research
Publication date: Available online 7 October 2019Source: Pathology - Research and PracticeAuthor(s): Dominik Gross, Christina LaursAbstractThe Jewish pathologist Carl Julius Rothberger (1871–1945) is undoubtedly one of the most important representatives of his field. His studies on atrial fibrillation, the bundle branch block and arrhythmia perpetua in particular secured him a place in medical history. Rothberger also gave the name to an agar used to prove the neutral red reduction of salmonella (Rothberger-Scheffler agar).While Rothberger’s name is well known in pathology, his biography and his experiences of ...
Source: Pathology Research and Practice - Category: Pathology Source Type: research
CONCLUSIONS: We found greater BV after training throughout spring/summer conditions, compared with interrupting spring/summer exposure to train at altitude in the cold, with no additional benefits observed from a heat camp following spring/summer training. PMID: 31621644 [PubMed - as supplied by publisher]
Source: International Journal of Sports Physiology and Performance - Category: Sports Medicine Tags: Int J Sports Physiol Perform Source Type: research
CONCLUSIONS: The heart rate recovery test, countermovement jump test, eccentric hamstring force test, and perceptual wellness all possess acceptable measurement sensitivity. Signal to noise ratio analysis is a novel method of assessing measurement characteristics of monitoring tools. These data can be used by coaches and scientists to identify meaningful changes in common measures of fitness and fatigue in professional Australian football. PMID: 31615972 [PubMed - as supplied by publisher]
Source: International Journal of Sports Physiology and Performance - Category: Sports Medicine Tags: Int J Sports Physiol Perform Source Type: research
Authors: Pincus T, Castrejon I, Yazici Y, Gibson KA, Bergman MJ, Block JA Abstract Osteoarthritis (OA) may be associated with substantial work disability, morbidity, costs, and increased mortality rates, often similar to rheumatoid arthritis (RA), documented in many published reports over the last 4 decades. However, OA generally has been viewed as less severe than RA. This discrepancy may be explained in part by:a) RA may have been considerably more severe in the past, prior to effective therapies.b) most older individuals have radiographic joint damage, which often is not associated with clinical symptoms.c) RA i...
Source: Clinical and Experimental Rheumatology - Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research
Authors: Griffin TM, Scanzello CR Abstract Although osteoarthritis (OA) was historically referred to as the non-inflammatory arthritis, it is now considered a condition involving persistent low-grade inflammation and activation of innate inflammatory pathways. Synovitis increases the risk of OA onset and progression and involves the recruitment of monocytes, lymphocytes, and other leukocytes. In particular, macrophages are important mediators of synovial inflammatory activity and pathologic cartilage and bone responses that are characteristic of OA. Advances in understanding how damage-associated molecular patterns...
Source: Clinical and Experimental Rheumatology - Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research
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