The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae

This study establishes RstAB as a major regulator of virulence and diverse cellular functions in P. damselae subsp. damselae. Introduction Two-component signal transduction systems enable bacteria to sense environmental stimuli and transfer this information across the cytoplasmic membrane to the cytoplasm (Stock et al., 2000). Such systems consist of a membrane-embedded protein kinase which acts as a sensory component, and its cognate response regulator, a cytoplasmic transcriptional factor. When the sensory component of the pair is stimulated by a specific signal, it autophosphorylates a histidine residue and then transfers the phosphate group to a conserved aspartate residue of the response regulator. Photobacterium damselae subsp. damselae (hereafter Pdd) is a marine bacterium pathogenic for a variety of marine animals as well as for humans, and represents an emerging threat for fish species of financial importance in marine aquaculture (Rivas et al., 2013a; Terceti et al., 2016; Osorio et al., 2018). pPHDD1 plasmid encodes two major virulence factors, the phospholipase-D damselysin (Dly) and the pore-forming toxin phobalysin P (PhlyP) (Rivas et al., 2011; Rivas et al., 2015a). Additional virulence factors are encoded within the chromosomes and include the pore-forming toxin phobalysin C (PhlyC), the phospholipase PlpV and the collagenase ColP (Rivas et al., 2013b; Vences et al., 2017). While production of PhlyC and PlpV are almost ubiquitous traits in this subspe...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 18 July 2019Source: Small Ruminant ResearchAuthor(s): J.M. González, T. Navarro, D. Lacasta, J.J. Ramos, L.M. Ferrer, N.G.C. Vasileiou, M. de las HerasABSTRACTVaccination programs against Ovine Respiratory Complex (ORC) in lambs should be accompanied by non-specific preventive measure, e.g., establishment of proper management and husbandry conditions. Implementing a vaccination plan against ORC requires: involvement of all farm staff, recording data, a positive cost:benefit analysis and continuous monitoring over time. Licensed vaccines for sheep are only against Mannheimia haemoly...
Source: Small Ruminant Research - Category: Zoology Source Type: research
Publication date: Available online 19 July 2019Source: Veterinary ParasitologyAuthor(s): Juan Pablo Ek-Huchim, Isabel Jiménez-García, Rossanna Rodríguez-CanulAbstractMonogeneans Gyrodactylus von Nordmann 1832, cause outbreaks of gyrodactylosis in aquaculture settings worldwide. Detection of Gyrodactylus spp. is based on the morphological identification of isolated parasites after fish necropsy. Contributing to the diagnosis of gyrodactylosis, in this study, a non-destructive PCR assay was standardized; the PCR was first performed using genomic DNA of Gyrodactylus spp. isolated from the surface of the N...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
Publication date: Available online 19 July 2019Source: Veterinary MicrobiologyAuthor(s): Milan Obradovic, J. Alex Pasternak, Siew Hon Ng, Brenda Allan, Robert Brownlie, Heather L. WilsonAbstractLawsonia intracellularis is an obligate intracellular microorganism and the causative agent of porcine proliferative enteropathy. Due to its obligate intracellular nature, characterization of antigens and proteins involved in host-pathogen interaction and immune recognition have been difficult to achieve using conventional microbiological techniques. In this work, we used 2-dimensional gel electrophoresis coupled with Western-immuno...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
This study aimed to investigate risk factors for canine obesity in adult companion dogs across Zealand, Denmark.Client-owned dogs (>2 years of age and without chronic illness) were recruited and examined at eight companion animal veterinary practices in areas with varying socio-economic characteristics. The body condition score (BCS) of the dogs was examined by two investigators based on a 9-point scoring scheme. Dog owners answered a questionnaire that had prompts regarding: 1) dog characteristics, including neuter status, 2) owner characteristics, 3) feeding and exercise practices and 4) the owners’ attachment t...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
Publication date: Available online 18 July 2019Source: Journal of Microbiological MethodsAuthor(s): Ming-An Tsai, Pei-Chi Wang, Shiomi Yoshida, Akio Aono, Satoshi Mitarai, Takayuki Wada, Shih-Chu ChenAbstractMycobacterium marinum is a zoonotic pathogen that can cause dermatological infection mainly from contaminated water or fish. Some well-known genetically similar species and subspecies are M. lifrandii and M. pseudoshottsii from amphibians and fish in aquaculture, and M. ulcerans, a causative agent of a neglected tropical disease (NTD), Buruli ulcer. They are believed to survive in water as their major niche, which migh...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
Publication date: Available online 18 July 2019Source: Radiation Physics and ChemistryAuthor(s): Rafael Molaria, Carlos RobertoAbstractLucas Cranach the Elder is one of the most important German painters of the XV and XVI centuries. It is presented and discussed the technical examination of The Portrait of a Young Gentleman, a wood panel from the collection of São Paulo Museum of Art (MASP) painted by Lucas Cranach the Elder in 1539. In situ PXRF and laboratory TXRF were employed for the elemental characterization of the pigments. The results allowed us to characterize the materials present in the original painting ...
Source: Radiation Physics and Chemistry - Category: Physics Source Type: research
Authors: Łabuz-Roszak B, Niewiadomska E, Kubicka-Bączyk K, Skrzypek M, Tyrpień-Golder K, Majewska A, Matejczyk A, Dobrakowski P, Pierzchała K Abstract OBJECTIVES: Pain is one of the most frequently reported symptoms of multiple sclerosis (MS). It affects the daily functioning of patients, limits the ability to work and reduces the joy of life. The aim of the study was to analyze the impact of pain on quality of life as well as symptoms of anxiety and depression in patients with MS. METHODS: The study included 144 patients with diagnosed MS (mean age 41±12 years, mean illness duration 10.3±8.6 ...
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
Authors: Herman A Abstract In recent years increased interest in the connection of intestinal microflora and the state of human health resulted in a great deal of research on the influence of intestinal microflora in particular on mental health, including works on affective functions of the central nervous system (CNS). Previous studies on animals have revealed the existence of a bi-directional communication system between the gastrointestinal microbiota and the central nervous system, the so-called .gut-brain axis' which modulates functioning of CNS through immunological, endocrine and neuronal mechanisms. Clinica...
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
CONCLUSIONS: On the basis of the study, it was indicated that the group of the highest risk are students of junior secondary school, probably because of the great interest in physical attractiveness in this period of life, as well as individuals with higher BMI. We believe that for more effective diagnosis it would be advisable to adopt a cut-off point for orthorexia in the ORTO-15 at the level of 35 points, as postulated by Stochel, Janas-Kozik et al. The 40-point threshold is associated with considerable overdiagnosis of the phenomenon. PMID: 31317965 [PubMed - in process]
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
CONCLUSIONS: 1) More than . of studied adolescents realize that there is no integrated system of suicide prevention; 2) The family, according to high school students, plays the greatest role in preventing self-destructive behaviors; 3) There is a need of greater involvement of schools, the Church, the police, health care institutions and the media in education and raising adolescents' awareness in the field of self-destructive behaviors.`. PMID: 31317963 [PubMed - in process]
Source: Psychiatria Polska - Category: Psychiatry Tags: Psychiatr Pol Source Type: research
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