Suppression of Conditional TDP-43 Transgene Expression Differentially Affects Early Cognitive and Social Phenotypes in TDP-43 Mice

This study was carried out in accordance with the recommendations of the National Animal Care and Use Committee of the University of Buenos Aires (CICUAL). The protocol was approved by the CICUAL. Mice were kept under a 12-h light/dark cycle, with controlled temperature (23 ± 2°C) and humidity (40–60%) and had ad libitum access to food and water. To produce hTDP-43 transgenic lines, as described previously (Igaz et al., 2011), pronucleus of fertilized eggs from C57BL/6J × C3HeJ F1 matings were injected with a vector containing hTDP-43-WT cDNA. Monogenic tetO-TDP-WT12 mice were bred to Camk2a-tTA mice (Mayford et al., 1996; Jackson Laboratory) generating non-transgenic, tTA monogenic, single tetO-TDP-43 transgenic mice (non-TDP-43 expressing control mice) and bigenic mice expressing hTDP-43-WT12 (hereinafter referred to as tTA/WT12). Mice were treated with 0.2 mg/ml Dox (Doxycycline Hyclate, sc-204734A, Santa Cruz Biotechnology) in drinking water to avoid prenatal and postnatal developmental effects of transgene expression. hTDP-43 expression was induced by switching mice to regular drinking water (without Dox) at weaning (postnatal day 28). Mice were analyzed at different time points (Figure 1A). FIGURE 1 Figure 1. TDP-43 expression pattern in inducible TDP-43-WT transgenic mice. (A) Experimental design: transgene expression was inactive until postnatal day 28 by treatment with Dox. For the suppression protocol, m...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research

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Source: Social Science and Medicine - Category: Psychiatry & Psychology Source Type: research
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Source: Computers in Human Behavior - Category: Information Technology Source Type: research
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People drinking this common beverage lost twice as much weight. → Support PsyBlog for just $4 per month. Enables access to articles marked (M) and removes ads. → Explore PsyBlog's ebooks, all written by Dr Jeremy Dean: Accept Yourself: How to feel a profound sense of warmth and self-compassion The Anxiety Plan: 42 Strategies For Worry, Phobias, OCD and Panic Spark: 17 Steps That Will Boost Your Motivation For Anything Activate: How To Find Joy Again By Changing What You Do
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Source: Journal of Anxiety Disorders - Category: Psychiatry Source Type: research
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Source: NaturalNews.com - Category: Consumer Health News Source Type: news
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Source: BBC News | Health | UK Edition - Category: Consumer Health News Source Type: news
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Source: NaturalNews.com - Category: Consumer Health News Source Type: news
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Source: Health News from Medical News Today - Category: Consumer Health News Tags: Alzheimer's / Dementia Source Type: news
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