Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance

A point-of-care HIV-1 genotypic resistance assay that could be performed during a clinic visit would enable care providers to make informed treatment decisions for patients starting therapy or experiencing virological failure on therapy. The main challenge for such an assay is the genetic variability at and surrounding each drug-resistance mutation (DRM). We analyzed a database of diverse global HIV sequences and used thermodynamic simulations to design an array of surface-bound oligonucleotide probe sets with each set sharing distinct 5 ’ and 3’ flanking sequences but having different centrally located nucleotides complementary to six codons at HIV-1 DRM reverse transcriptase position 103: AAA, AAC, AAG, AAT, AGA, and AGC.

Source: Journal of Molecular Diagnostics - April 22, 2019 Category: Pathology Authors: Dana S. Clutter, Gelareh Mazarei, Ruma Sinha, Justen Manasa, Janin Nouhin, Ellen LaPrade, Sara Bolouki, Philip L. Tzou, Jessica Hannita-Hui, Malaya K. Sahoo, Peter Kuimelis, Robert G. Kuimelis, Benjamin A. Pinsky, Gary K. Schoolnik, Arjang Hassibi, Robert Tags: Regular Article Source Type: research