Da-Bu-Yin-Wan and Qian-Zheng-San Ameliorate Mitochondrial Dynamics in the Parkinson ’s Disease Cell Model Induced by MPP+

Discussion In this experiment, the selected SH-SY5Y cell strain of human neuroblastoma is characterized by its cell morphology, physiology and biochemical characteristics similar to normal neurons, rapid reproduction, and many properties of dopaminergic neurons. Therefore, the cell strain is widely used in vitro studies of neurological diseases, especially in PD (Feng et al., 2006; Xie et al., 2010). MPP+ is a neurotoxin that has often been used to establish experimental cell model of PD. Studies showed that MPP+ inhibited the activity of mitochondrial complex I, interfered with oxidative phosphorylation, and reduced the synthesis of ATP, thus interrupted the normal operation of the mitochondrial respiratory chain, leading to excessive fragmentization of mitochondria, lack of ATP, loss of mitochondrial membrane potential, and eventually cell death (Zeng et al., 2017; Zhang et al., 2017; Zhong et al., 2018). In this experiment, MPP+ was selected to construct the PD cell model. Our experimental results were consistent with the former literature reports, suggesting our successful modeling. In recent decades, DBYW and QZS were clinically employed to treat PD. Clinical statistics found that the treatment of PD with traditional Chinese formulas in 189 articles, a total of 110 kinds of Chinese medicinals were involved, among which the Chinese medicinals for nourishing the liver and the kidney: shú dì huáng was used for 51 times accounting for 2.6%, guī...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research