Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs

This study was supported by NIH/NIAID grants R01AI102766 (YL), P01AI104722 (RW and YL), UM1 AI100663 (RW), and Bill and Melinda Gates Foundation grant OPP1084519 (RW). JS is a trainee of NIH training grant T32AI125186A to Anne Simon at University of Maryland, College Park. This study was also partially funded by the International AIDS Vaccine Initiative (IAVI) (RW) with the generous support of United States Agency for International Development (USAID), and Ministry of Foreign Affairs of the Netherlands. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Part of this work took place at Covance as a fee-for-service. Covance had no involvement with the study. Acknowledgments We thank Heather Arendt and Joanne DeStefano for coordinating the immunogenicity study. Abbreviations bNAbs, broadly neutralizing antibodies; BLI, BioLayer Interferometry; CDR3, complementarity-determining region; D, diversity; ELISA, enzyme-linked immunosorbent assay; FACs, fluorescence-activated cell sorting; Ig, immunoglobulin; IGH, Ig heavy; IGL, Ig lambda; IGK, Ig kappa; J, joining; mAb, monoclonal antibodies; PBMCs, peripheral blood mononuclear cells; V, variable. Footnotes ^http://www.ensembl.org References Alamyar, E., Giudicelli, V., Li, S., Duroux, P., and Lefranc, M. P. (2012). IMGT/Highv-quest: the IMGT® web portal for imm...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research