Development of a Blocking Primer to Inhibit the PCR Amplification of the 18S rDNA Sequences of Litopenaeus vannamei and Its Efficacy in Crassostrea hongkongensis

Conclusion Predator–prey interactions in marine ecosystems are of critical importance in the structuring of marine communities and determining the health of the world’s oceans (Bailey et al., 2010). The host-specific blocking primer against L. vannamei, X-BP2-DPO is a highly promising tool to inhibit the 18S rDNA amplification of this shrimp species. Addition of the blocking primer could greatly enhance the quantity and the diversity of the eukaryotic microorganisms in shrimps, thereby improving our ability to maximize the gut content information about them in favor of studying the taxa composition and their relationships with environment. The development of blocking primers will offer more universal applicability to different marine animals based on the guarantee of simplicity and efficiency of the blocking primer against specific species. Ethics Statement All animal work have been conducted according to relevant national and international guidelines. South China Sea Fisheries Research Institute Academic Committee approved this research. Author Contributions CL, R-JQ, and J-ZJ conceived and planned the experiments. CL, R-JQ, and M-QZ carried out the experiments. CL analyzed the sequencing data. J-ZJ and J-YW helped to supervise the experiments and analysis. CL and J-ZJ wrote the manuscript. All the authors provided critical feedback and helped to shape the research, analysis and manuscript. Funding This work was supported by the Central Public-interest S...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research