Shengui Sansheng San Ameliorates Cerebral Energy Deficiency via Citrate Cycle After Ischemic Stroke

Conclusion In summary, SSS extraction significantly ameliorates cerebral energy metabolism via boosting citrate cycle, which mainly embodies the enhancements of blood glucose concentration, glucose and lactate transportation and glucose utilization, as well as the regulations of relative enzymes activities in citrate cycle. These ameliorations ultimately resulted in numerous ATP yield after stroke, which improved neurological function and infarcted volume. Collectively, it suggests that SSS extraction has exerted advantageous effect in the treatment of cerebral ischemia. Ethics Statement All animal operations were accorded to Guide for the Care and Use of Laboratory Animals (8th edition, Washington, DC, United States: The National Academies Press). The experimental protocol was approved by Macau University of Science and Technology ethical committee and every experimental step of the study was performed in accordance with the regulations of animals used in research projects issued by Instituto para os Assuntos Cívicos e Municipais of Macao Special Administrative Region. Author Contributions YZ and J-LW designed the research. CL, ZX, and BL evaluated neurological function, performed western blot, immunofluorescence staining, and ELISA in animals experiments. XB completed quality control and performed DIAAA Derivatization-UHPLC-Q-TOF/MS test. QZ and CK finished MRI and MicroPET/CT detections and analysis. QC tested the blood glucose level. CL and XB wrote the manusc...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research