A low-cost microfluidic platform for rapid and instrument-free detection of whooping cough.

A low-cost microfluidic platform for rapid and instrument-free detection of whooping cough. Anal Chim Acta. 2019 Aug 13;1065:71-78 Authors: Dou M, Sanchez J, Tavakoli H, Gonzalez JE, Sun J, Dien Bard J, Li X Abstract Whooping cough also called Pertussis is a highly contagious respiratory infection that affects all age populations. Given recent pertussis outbreaks, there is an urgent need for a point-of-care (POC) device for rapid diagnosis of pertussis. Herein, we report a low-cost microfluidic POC device integrated with loop-mediated isothermal amplification (LAMP) technique for the rapid and accurate diagnosis of pertussis. The 3D-printed bioanalyzer housed not only the biochip but also an in-house-developed portable and fully battery-powered heater for rapid POC detection of pertussis, without the need of external electricity. The fluorescence-based results could be rapidly visualized in about one hour by the naked eye without the need for any additional instrumentation. In addition, a simple centrifuge-free sample preparation process was optimized for the efficient lysis of pertussis samples and successfully used for direct detection of bacteria in nasopharyngeal samples. High sensitivity, with a limit of detection (LOD) of 5 DNA copies per LAMP zone, and high specificity were demonstrated. We envision that the microfluidic POC device can be used in various venues such as medical clinics, schools, and other low-resource settings for the fast detectio...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research

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[Vaccine rejection and vaccination management: the grey areasRecusa vacinal e gestão da imunização: nuances e contrastes]. Rev Panam Salud Publica. 2019;43:e54 Authors: Hortal M, Di Fabio JL Abstract Vaccinating children has been an unquestioned tradition for many years. However, there is now great concern over the growing rejection of childhood vaccination, as well as other less evident obstacles that affect vaccination coverage.Multiple factors are involved in the rejection of a specific vaccine or vaccination in general, including actions by anti-vaccination groups, as well as d...
Source: Pan American Journal of Public Health - Category: International Medicine & Public Health Tags: Rev Panam Salud Publica Source Type: research
Christina Hildebrand went down a rabbit hole and emerged at the statehouse in Sacramento. That’s how she describes it–going down a rabbit hole–and in her case it happened 14 years ago, when she was pregnant with her first child. In a world filled with chemicals and toxins, processed foods and GMOs, she decided her baby would be brought up as naturally and chemical-free as possible. It was when she was researching how best to achieve that goal that she bumped into vaccines. That was a bad time to begin thinking about such things. The fraudulent 1998 paper by British physician Andrew Wakefield ostensibly li...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized vaccines Source Type: news
MONDAY, June 10, 2019 -- The waning effectiveness of a flawed whooping cough vaccine is the main culprit in recent outbreaks of the highly contagious bacterial infection, a new study reports. More than four out of five confirmed whooping cough...
Source: Drugs.com - Daily MedNews - Category: General Medicine Source Type: news
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Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Maine onetime vaccinations Source Type: news
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Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized measles onetime washington Source Type: news
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Source: The Medical Futurist - Category: Information Technology Authors: Tags: Empowered Patients Future of Medicine anti-vaccination anti-vax anti-vaxxer digital disease disease outbreak facebook figth Health Healthcare infection Innovation measles movement social media technology Source Type: blogs
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
A whooping cough outbreak has been declared in Fredericton, where 10 children from "several" schools and two adults have been diagnosed since January, the regional medical officer of health announced Wednesday.
Source: CBC | Health - Category: Consumer Health News Tags: News/Canada/New Brunswick Source Type: news
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