Nicotine Inhibits Rapamycin-induced Pain through Activating mTORC1/S6K/IRS-1-related Feedback Inhibition Loop.

In this study, pain was induced in naïve male C57BL/6 J mice by intraperitoneally injecting rapamycin acutely or repeatedly. Subsequently, pain thresholds, including mechanical and thermal pain, were measured. The involving signaling pathway was tested using western blot analysis and immunofluorescent assay. Changes in neuronal excitability caused by different treatments were also analyzed using whole-cell recording. Microinjection into the anterior cingulate cortex (ACC) was used to test the role of nAChRs containing the α4β2 or α7 subtype in this brain region in pain modulation. Our results showed that nicotine significantly reduced hyperalgesia in mice that received acute or repeated rapamycin injections, and reversed the effects of rapamycin on the phosphorylation of S6K, 4E-BP1, insulin receptor substrate-1 (IRS-1) at Ser636/639, AKT at Ser473, and ERK at Thr202/Tyr204. Whole-cell recording results showed that nicotine reduced the firing rates of pyramidal neurons in the ACC, and a pharmacological blockade of nAChRs containing the α4β2 or α7 subtype in ACC inhibited the antinociceptive effects of nicotine in mice with rapamycin-induced pain. Our findings indicate that analgesics targeting nAChRs can be developed to help patients with rapamycin-induced pain. PMID: 31005665 [PubMed - as supplied by publisher]
Source: Brain Research Bulletin - Category: Neurology Authors: Tags: Brain Res Bull Source Type: research

Related Links:

Chest pain is a frequent reason for calls in emergency medical communication centre (EMCC). Detecting a coronary origin by phone is a challenge. This is especially so as the presentations differ according to g...
Source: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine - Category: Emergency Medicine Authors: Tags: Original research Source Type: research
ConclusionUnnecessary procedures to the proximal biceps, aiming to prevent chronic pain, should be avoided in fracture fixation as they would affect a significant source of blood supply to the humeral head. These findings could help surgeons protect the only vascular supply they can during the fixation of proximal humeral fractures, when using the anterior or antero-lateral approaches.
Source: Surgical and Radiologic Anatomy - Category: Anatomy Source Type: research
ConclusionsThe study demonstrates that the proposed body-mounted robotic system is able to perform MRI-guided low back injection in a phantom study with sufficient accuracy and with minimal visible image degradation that should not affect the procedure.
Source: International Journal of Computer Assisted Radiology and Surgery - Category: Intensive Care Source Type: research
In this issue, Tang et al. [1] from Taiwan report on an increasingly important issue in thoracic medicine and surgery, the natural history of small, asymptomatic lung nodules. The widespread use, over the last couple of decades, of increasingly sensitive computed tomography (CT) scans with reducing radiation dose has resulted in the recognition of a significant population of otherwise healthy patients who have a lung nodule of undefined nature. In most cases, this lung nodule has been detected at the time of a CT scan for a completely different issue —atypical chest pain, back pain, shoulder pain, and often for inves...
Source: Heart, Lung and Circulation - Category: Cardiology Authors: Tags: Editorial Source Type: research
ConclusionGU and GI fistulas are rare but morbid entities, which most often manifest after missed bladder or bowel injuries, post benign gynecological surgeries. Early detection and recognition of GI and GU injuries may be key for secondary prevention of fistula formation.
Source: Journal of Minimally Invasive Gynecology - Category: OBGYN Source Type: research
X-ray imaging shows that corticosteroid injections for osteoarthritis pain...Read more on AuntMinnie.comRelated Reading: 3D MRI helps predict osteoarthritis risk after ACL tears 3 ways to reduce unnecessary MRI knee scans 6 ways AI will enhance musculoskeletal imaging Many knee MRI scans may not be clinically indicated New 3D CT technique tops x-ray for diagnosing arthritis
Source: Headlines - Category: Radiology Source Type: news
ConclusionsThis case report demonstrates an unusual etiology of back and leg pain in a young man and elucidates the palliative effects of radiation therapy for epithelioid hemangioma involving the lumbar spine.
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
AbstractA 45 ‐year‐old male presented in decompensated heart failure from nonischemic cardiomyopathy and was implanted with a right transaxillary intra‐aortic balloon pump. He was listed for heart transplantation but after 9 days, he developed abdominal pain with evidence of embolization on computed tomogr aphy scan despite a well‐positioned balloon pump. He underwent concomitant abdominal exploration with small bowel resection and durable left ventricular assist device implantation. He recovered well and was discharged home. This case highlights the challenges of decision‐making in the current er a where intra...
Source: Journal of Cardiac Surgery - Category: Cardiovascular & Thoracic Surgery Authors: Tags: CASE REPORT Source Type: research
AbstractIntroductionSurgery by open radical hysterectomy for cervical cancer is associated with sexual dysfunction as well as lymphedema and bladder problems. Our aim was to assess the impact of robot ‐assisted laparoscopic radical hysterectomy (RRH) with pelvic lymphadenectomy for early‐stage cervical cancer on sexual, bowel, bladder, and lymphatic function and to measure ovarian function after RRH.Material and methodsTwenty ‐six women with early‐stage cervical cancer during 2011‐2013 were investigated before and 1 year after RRH using a validated questionnaire measuring psychological well‐being and sexua...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
AbstractIntroductionDespite the associations between delayed childbearing and poorer maternal and perinatal outcomes, little is known about these issues in regular exercisers and in women with healthy lifestyles. The aims of the present study were to: (a) compare lifestyle variables and exercise, pregnancy and birth outcomes in women ≥35 years and women
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
More News: Brain | Insulin | Neurology | Neuroscience | Nicotine | Pain | PET Scan | Science | Study