Removal of Cell Wall Polysaccharide in Pneumococcal Capsular Polysaccharides by Selective Degradation via Deamination

Publication date: Available online 20 April 2019Source: Carbohydrate PolymersAuthor(s): Wei Zou, Jianjun Li, Evguenii Vinogradov, Andrew CoxAbstractPneumococcal cell wall polysaccharide (C-PS), a contaminant in pneumococcal capsular polysaccharide (Pn-PS) vaccines is degraded by mild deamination of the 4-amino-2-acetamido-2,4,6-tri-deoxy-galactose (AAT) in C-PS, which was carried out by addition of 5% aqueous sodium nitrite to a solution of polysaccharide in 5% aqueous acetic acid. Glycosidic linkage and functional groups such as O-acetates, phosphodiesters, and pyruvates were preserved under the conditions. The small fragments from degraded C-PS were removed by ultrafiltration or dialysis to provide essentially C-PS free Pn-PS. Because of the presence of AAT in its structure the deamination is not suitable for the purification of type 1 Pn-PS. Meanwhile, the mass and NMR spectroscopic analysis on the deamination products suggests that both type 1 Pn-PS and C-PS degraded following a major pathway of 5,4-hydride shift, cleavage of AAT O5-C1 bond, C1 hemiacetal formation, and its hydrolysis to release neighboring GalA- in type 1 Pn-PS and GalNAc(6-O-PCho)- in C-PS.
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research

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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: The American Journal of Managed Care - Category: Health Management Authors: Tags: Am J Manag Care Source Type: research
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Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
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Source: American Journal of Preventive Medicine - Category: Global & Universal Source Type: research
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Source: Clinical and Vaccine Immunology - Category: Allergy & Immunology Authors: Tags: Clin Vaccine Immunol Source Type: research
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