Titania nanotubes promote osteogenesis via mediating crosstalk between macrophages and MSCs under oxidative stress

Publication date: Available online 18 April 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Xinkun Shen, Yonglin Yu, Pingping Ma, Zhong Luo, Yan Hu, Menghuan Li, Ye He, Yangyang Zhang, Zhihong Peng, Guanbin Song, Kaiyong CaiAbstractBefore mesenchymal stem cells (MSCs) adhere to the surface of an implant and differentiate into osteoblasts, monocytes (especially macrophages) arrive at the bone injury site and interact with the implant and subsequent MSCs. In our previous study, large titania nanotubes (TNT110) had been verified to endow superior oxidation resistance to osteoblasts. The early regulation between macrophages and MSCs by surface nanotubes under oxidative stress (OS) was evaluated further in this study. Cellular and molecular results show that TNT110 greatly increased early inflammation of macrophages by activating integrin/FAK-mediated MAPK and NFκB signals and simultaneously promoted their gene expression of SDF1, IL-8, and CCL2 (chemokines) compared with 30 nm nanotubes and titanium substrates. Co-culture results show that more MSCs were recruited by those chemokines and may promote osteogenic self-differentiation, reducing early inflammation of macrophages by accelerating their M1-to-M2 transition in the TNT110 group. All findings reveal that the early cellular behavior of macrophages and MSCs was effectively regulated by TNT110, indicating large nanotubes would be more suitable to prevent oxidative damages.Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research