Molecules, Vol. 24, Pages 1570: Impact of Small Molecules on Intermolecular G-Quadruplex Formation

Molecules, Vol. 24, Pages 1570: Impact of Small Molecules on Intermolecular G-Quadruplex Formation Molecules doi: 10.3390/molecules24081570 Authors: Prabesh Gyawali Keshav GC Yue Ma Sanjaya Abeysirigunawardena Kazuo Nagasawa Hamza Balci We performed single molecule studies to investigate the impact of several prominent small molecules (the oxazole telomestatin derivative L2H2-6OTD, pyridostatin, and Phen-DC3) on intermolecular G-quadruplex (i-GQ) formation between two guanine-rich DNA strands that had 3-GGG repeats in one strand and 1-GGG repeat in the other (3+1 GGG), or 2-GGG repeats in each strand (2+2 GGG). Such structures are not only physiologically significant but have recently found use in various biotechnology applications, ranging from DNA-based wires to chemical sensors. Understanding the extent of stability imparted by small molecules on i-GQ structures, has implications for these applications. The small molecules resulted in different levels of enhancement in i-GQ formation, depending on the small molecule and arrangement of GGG repeats. The largest enhancement we observed was in the 3+1 GGG arrangement, where i-GQ formation increased by an order of magnitude, in the presence of L2H2-6OTD. On the other hand, the enhancement was limited to three-fold with Pyridostatin (PDS) or less for the other small molecules in the 2+2 GGG repeat case. By demonstrating detection of i-GQ formation at the single molecule level, our studies illustrate the feasibi...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research