Expanding Phaseolus coccineus Genomic Resources: De Novo Transcriptome Assembly and Analysis of Landraces 'Gigantes' and 'Elephantes' Reveals Rich Functional Variation.
Expanding Phaseolus coccineus Genomic Resources: De Novo Transcriptome Assembly and Analysis of Landraces 'Gigantes' and 'Elephantes' Reveals Rich Functional Variation.
Biochem Genet. 2019 Apr 17;:
Authors: Xanthopoulou A, Kissoudis C, Mellidou I, Manioudaki M, Bosmali I, Tsakonitis V, Kalivas A, Osathanunkul M, Tsaftaris A, Ganopoulos I, Madesis P
Abstract
Beans are one of the most important staple crops in the world. Runner bean (Phaseolus coccineus L.) is a small-scale agriculture crop compared to common bean (Phaseolusvulgaris). Beans have been introduced to Europe from the Central America to Europe and since then they have been scattered to different geographical regions. This has resulted in the generation of numerous local cultivars and landraces with distinguished characters and adaptive potential. To identify and characterize the underlying genomic variation of two very closely related runner bean cultivars, we performed RNA-Seq with de novo transcriptome assembly in two landraces of P. coccineus, 'Gigantes' and 'Elephantes' phenotypically distinct, differing in seed size and shape. The cleaned reads generated 37,379 and 37,774 transcripts for 'Gigantes' and 'Elephantes,' respectively. A total of 1896 DEGs were identified between the two cultivars, 1248 upregulated in 'Elephantes' and 648 upregulated in 'Gigantes.' A significant upregulation of defense-related genes was observed in 'Elephantes,' among those, numerous members...
Source: Biochemical Genetics - Category: Genetics & Stem Cells Authors: Xanthopoulou A, Kissoudis C, Mellidou I, Manioudaki M, Bosmali I, Tsakonitis V, Kalivas A, Osathanunkul M, Tsaftaris A, Ganopoulos I, Madesis P Tags: Biochem Genet Source Type: research