053 Molecular mechanism of miR-145/Smad3 pathway mediated by active vitamin D in Lupus Nephritis

To elucidate the roles of 1,25(OH)2D3 and VDR in the pathogenesis of Lupus Nephritis (LN) by regulating miR-145 expression through TGF- β1-dependent Smad3 signaling pathway.From January 1, 2012 to January 1, 2017, 49 children (11 males and 38 females) with LN and 50 healthy controls were selected in this study. CD4+Tcells treated with different concentrations of 1,25(OH)2D3(1×10-6mmol/L,1×10-7mmol/L,1×10-8mmol/L).VDR-siRNA inter ference vector and human VDR overexpression vector were constructed. CD4+T cells in logarithmic growth phase were divided into 6 groups: the normal control group, the normal negative control (NC) group, the VDR siRNA group, the LN control group, the LN NC group, and the VDR over-expressed LN group.S erum level of 1,25(OH)2D3 was detected by ELISA, The mRNA and protein expressions of VDR, miR-145,TGF-β1 and Smad3,pSmad3 were detected by RT-qPCR and Western blotting.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Adaptive and Auto-Immunity Source Type: research