Diminished cytolytic activity of γδ T cells with reduced DNAM-1 expression in neuroblastoma patients.

Diminished cytolytic activity of γδ T cells with reduced DNAM-1 expression in neuroblastoma patients.
Clin Immunol. 2019 Apr 15;:
Authors: Wang X, Mou W, Han W, Xi Y, Chen X, Zhang H, Qin H, Wang H, Ma X, Gui J
Abstract
Neuroblastoma is one of the children's malignant tumors with poor prognosis, as well as high recurrence and metastasis rates after surgical removal and chemotherapy. γδ T-cell based immunotherapy receives increasing attention thanks to their strong cytolytic activity to tumor cells. Our previous data revealed a significant increase in circulating γδ T-cell frequency in NB patients. In the present study, we found that beside a reduction of IFN-γ in serum of NB patients, DNAM-1 expression decreased in both circulating and PAM-expanded NB γδ T cells. Upon PAM stimulation, NB γδ T cells showed a reduced level of cell proliferation. In addition, the cytolytic activity of NB γδ T cells to NB cell lines was proved to be attenuated in a co-culture system. The fact that DNAM-1 neutralizing antibody abolished the tumor cell killing accentuates the indispensable role of DNAM-1 molecule in γδ T-cell cytolytic function.
PMID: 30999035 [PubMed - as supplied by publisher]

Source: Clinical Immunology - April 14, 2019 Category: Allergy & Immunology Authors: Wang X, Mou W, Han W, Xi Y, Chen X, Zhang H, Qin H, Wang H, Ma X, Gui J Tags: Clin Immunol Source Type: research