Quantitative proteomic analysis of a genetically induced prostate inflammation mouse model via custom 4-plex DiLeu isobaric labeling.

Quantitative proteomic analysis of a genetically induced prostate inflammation mouse model via custom 4-plex DiLeu isobaric labeling. Am J Physiol Renal Physiol. 2019 Apr 17;: Authors: Hao L, Thomas S, Greer T, Vezina CM, Bajpai S, Ashok A, De Marzo AM, Bieberich C, Li L, Ricke WA Abstract Inflammation is involved in many prostate pathologies including infection, benign prostatic hyperplasia (BPH), and prostate cancer (CaP). Preclinical models are critical to our understanding of disease mechanisms, yet few models are genetically tractable. Here we present a comparative quantitative proteomic analysis of urine from mice with and without prostate-specific inflammation induced by conditional prostate epithelial interleukin-1 beta (IL-1 beta) expression. Relative quantification and sample multiplexing was achieved using custom 4-plex N,N-dimethyl leucine (DiLeu) isobaric tags and nanoflow ultrahigh performance liquid chromatography coupled to high-resolution tandem mass spectrometry. Each set of 4-plex DiLeu reagents allows four urine samples to be analyzed simultaneously, providing high-throughput and accurate quantification of urinary proteins. Proteins involved in the acute phase response, including haptoglobin, inter-alpha-trypsin inhibitor, and alpha-1-antitrypsin 1-1, were differentially represented in urine of mice with prostate inflammation. Mass spectrometry-based quantitative urinary proteomics represents a promising bioanalyt...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research