Efficacy of Andrographis paniculata against AmpC producing multi drug resistant E. coli

Publication date: Available online 18 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Saroj Kumar Sah, Ubaid Rasool, D. Mubarak Ali, S. HemalathaAbstractAndrographis paniculata (Acanthaceae) is a well-known medicinal plant and is conventionally used in herbal medicine for the treatment of several diseases such as cancer, metabolic disorders, skin infections, respiratory tract infections, etc. In this present study, ethyl acetate extract of A. paniculata was used to test its efficacy on controlling growth and biofilm formation in multi drug resistant pathogenic strains of Escherichia coli through micro-dilution method. Antibiotic susceptibility screening through disc diffusion revealed that these E. coli strains were resistant towards 13 out of 15 antibiotics used. It was found that 250 and 500 μg/ml of crude ethyl acetate (CEA) extract effectively reduced the growth and biofilm formation as compared to 62 and 125 μg/ml. The strains treated with CEA extract of A. paniculata reduced extracellular polysaccharide (EPS) production and downregulated the expression of AmpC gene. The results suggested that CEA extract of A. paniculata can be explored to utilize as an alternative to antibiotics.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research