Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria

Publication date: Available online 14 October 2014 Source:Microbiological Research Author(s): Jana Klimentová , Jiří Stulík Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host–pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.
Source: Microbiological Research - Category: Infectious Diseases Source Type: research