Imatinib-induced changes in the expression profile of microRNA in the plasma and heart of mice—A comparison with doxorubicin

Publication date: July 2019Source: Biomedicine & Pharmacotherapy, Volume 115Author(s): Barbora Hanousková, Mikuláš Skála, Veronika Brynychová, Tomáš Zárybnický, Veronika Skarková, Petra Kazimírová, Andrea Vernerová, Pavel Souček, Lenka Skálová, Radek Pudil, Petra MatouškováAbstractCardiotoxicity is a serious adverse reaction to cancer chemotherapy and may lead to critical heart damage. Imatinib mesylate (IMB), a selective tyrosine kinase inhibitor, is sometimes accompanied by severe cardiovascular complications. To minimize risk, early biomarkers of such complications are of utmost importance. At the present time, microRNAs (miRNAs) are intensively studied as potential biomarkers of many pathological processes. Many miRNAs appear to be specific in some tissues, including the heart. In the present study we have explored the potential of specific miRNAs to be early markers of IMB-induced cardiotoxicity. Doxorubicin (DOX), an anthracycline with well-known cardiotoxicity, was used for comparison. NMRI mice were treated with IMB or DOX for nine days in doses corresponding to the highest recommended doses in oncological patients, following which plasmatic levels of miRNAs were analyzed in miRNA microarrays and selected cardio-specific miRNAs were quantified using qPCR. The plasmatic level of miR-1a, miR-133a, miR-133b, miR-339, miR-7058, miR-6236 and miR-6240 were the most different between the IMB-treated and control mice. Interestingly, most of the miRNAs affect...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research