Upregulation of miR-214 Induced Radioresistance of Osteosarcoma by Targeting PHLDA2 via PI3K/Akt Signaling

This study was carried out in accordance with the recommendations of international guidelines and ethical standards with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Institutional Ethics Committee of Kunming General Hospital, China. miRNAs, Plasmids, Transfection, and Irradiation To predict miRNAs with potential binding sites in the 3′UTR of PHLDA2, Targetscan (http://www.targetscan.org/), miRanda (http://www.microrna.org/), and Diana database (http://diana.cslab,ece.nura.gr/) algorithms were used. Mature hsa-miR-214, -134, -193a-3p, -376c, -425, or -499-5p mimics (double-stranded oligonucleotides designed to mimic as endogenous miRNA), anti-hsa-miR-214, or antagomir-214 (antisense RNA oligonucleotides to inhibit the function of miRNA), and individual scrambles (negative control) were purchased from RuiBoBio (Guangzhou, China). A final maximum effective concentration of mimics and inhibitor were 50 nM. The PHLDA2 CDS sequence without the 3′UTR was cloned into the pcDNA-3.0 plasmid by PCR amplification to construct the pcDNA-PHLDA2 vector as presented previously (8). Transfections were carried out with Lipofectamine 2000 (Invitrogen, USA). External beam radiation was delivered on a 300 KV X ray machine (HITACHI, Japan) at room temperature. Cells were pretreated with PI3K inhibitor LY294002 (Sigma, USA) for 1 h before irradiation. Target in v...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

Related Links:

ConclusionAccording Result this study the average concentration of isoflurane and the health risk assessment in Valiasr and Shahid Beheshti teaching hospital operating room during 2018 because of flaw in the ventilation system was significantly higher than standard.
Source: Clinical Epidemiology and Global Health - Category: Epidemiology Source Type: research
Authors: Friedrichs K, Rudolph V Abstract Percutaneous mitral valve repair with the MitraClip in the COAPT study significantly reduced overall mortality and hospitalization in patients with at least moderate to severe mitral valve regurgitation, in comparison to guideline-compliant drug treatment alone. Consequently, the assumption that secondary mitral regurgitation is more a consequence than the cause of systolic heart failure needs to be revised; however, data from the simultaneously published MITRA-FR study showed no prognostic benefits for patients with advanced heart failure and severely enlarged left ventric...
Source: Herz - Category: Cardiology Tags: Herz Source Type: research
CONCLUSION: LSS using three time-point (C1, C4, and C8) provides the most reliable and accurate simultaneous estimation of the AUC0-12 of MPA and AcMPAG in Japanese lung transplant patients. PMID: 31430249 [PubMed - in process]
Source: J Pharm Pharm Sci - Category: Drugs & Pharmacology Authors: Tags: J Pharm Pharm Sci Source Type: research
We present the second case of IDC originating from an intraparotid lymph node (LN) with a more detailed description of the histogenesis, immunohistochemistry (IHC) and updated molecular information. CASE REPORT: An 87-year-old male had a tumour nodule over the left parotid tail for about 20 years. Physical examinations revealed a 4.5 cm soft, non-tender and fixed mass. After the left parotidectomy, pathology confirmed the diagnosis of IDC arising within an intraparotid lymph node. The cystic component of the tumour was lined by single to multilayered ductal cells with micropapillary growth pattern. The solid part showe...
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
We present a case of an undifferentiated subtype of non-keratinizing squamous cell carcinoma (NK-SCC) with sarcomatoid features in the nasopharynx in a 69-year-old man who was difficult to diagnose due to spindle-shaped malignant cells. He was admitted because of a right nasal obstruction and right headache, and imaging revealed a heterogeneously enhanced irregularly shaped mass at the nasopharynx. Histopathologically, the tumour was partially organised, and the tumour cells were epithelioid or spindle-shaped. Initially, we erroneously diagnosed the tumour as an angiosarcoma owing to its false-negative immunoreaction for c...
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
We report a case of a 46-year-old Chinese male who presented with six months history of abdominal pain, weight loss and rectal bleeding. Diagnostic colonoscopy revealed multiple aphthous ulcers within the ileo-caecal region and distal transverse colon, separated by normal mucosa, mimicking skip lesions of Crohn's colitis. Computer topography (CT) scan of the abdomen showed multiple circumferential thickenings involving predominantly the right colon. A clinical diagnosis of colonic Crohn's disease with possible perforation was made. An extended right hemicolectomy was performed due to uncontrolled rectal bleeding. Histopath...
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
We report cytomorphological features of two uncommon variants of acinic cell carcinoma. The first case was an eleven-year-old female with a nodular mass in parotid and the FNA smears demonstrated a lymphoepithelial lesion composed of epithelial tumour cells with features of acinar cells in a lymphoid background. The second case was a 62-year-old male with a large parotid mass. The FNA smears revealed presence of extracellular, acellular amyloid-like material with tumour cells arranged in follicles. DISCUSSION: Awareness of cytomorphological features of these unusual variants of acinic cell carcinoma may help to avoid d...
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
In this study, we compared FISH with QRT-PCR for detection of residual disease in CML. MATERIALS AND METHODS: CML patients on tyrosine kinase inhibitor (TKI) therapy and on regular follow up at University Kebangsaan Malaysia Medical Center (UKMMC) were selected. A comparative study was conducted between FISH and QRT-PCR for BCR-ABL transcripts at diagnosis and during follow-up. RESULTS: There was good correlation between FISH and QRT-PCR for BCR-ABL. At 6th month of follow-up post diagnosis, FISH had a sensitivity of 83.3% and specificity of 65.2% (k>0.339, p0.286, p 0.642, p
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
Authors: Md Pauzi SH, Saari HN, Roslan MR, Syed Khair Azman Jamalulil SNS, Tauan IS, Mohd Rusli FA, Aizuddin AN Abstract INTRODUCTION: Evaluation of HER2 status in breast cancer using immunohistochemistry (IHC) and in-situ-hybridisation (ISH) study is important to establish prognosis and to select patient for targeted therapy. OBJECTIVE: The study aims to determine the concordance between HER2 protein IHC score and its gene status by dual-colour dual-hapten in-situ-hybridization (DDISH) study. MATERIALS AND METHODS: Retrospective study was performed on 767 referred breast cancer cases over a period of five ...
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
CONCLUSION: AR is expressed in about one-third of TNBC and loss of AR immunoexpression does not predict adverse clinical outcomes. Larger cohorts for better characterisation of the role of AR immunoexpression in TNBC are warranted. PMID: 31427547 [PubMed - in process]
Source: Malaysian Journal of Pathology - Category: Pathology Tags: Malays J Pathol Source Type: research
More News: Academia | Alcoholism | Biotechnology | Bone Cancers | Brain | Brain Cancers | Brain Tumor | Breast Cancer | Breast Carcinoma | Cancer | Cancer & Oncology | Cancer in Adolescents | Carcinoma | Chemistry | Chemotherapy | Children | China Health | Chondrosarcoma | Colon Cancer | Colorectal Cancer | Databases & Libraries | ENT & OMF | Esophagus Cancer | External Beam Therapy | Gastric (Stomach) Cancer | Gastroenterology | Gastroschisis Repair | Genetics | Grants | Japan Health | Legislation | Lung Cancer | Medical Ethics | Melanoma | Men | Milk | Molecular Biology | Nanotechnology | Neoadjuvant Chemotherapy Therapy | Neoadjuvant Radiation Therapy | Neoadjuvant Therapy | Neurology | Neuroscience | Neurosurgery | Osteosarcoma | Pancreas | Pancreatic Cancer | Pathology | Pediatrics | Penicillin | Radiation Therapy | Reproduction Medicine | Sarcomas | Science | Skin Cancer | South Korea Health | Squamous Cell Carcinoma | Statistics | Students | Study | Surgery | Switzerland Health | Translocation | Universities & Medical Training | Urology & Nephrology | USA Health