Upregulation of miR-214 Induced Radioresistance of Osteosarcoma by Targeting PHLDA2 via PI3K/Akt Signaling

Conclusions In summary, our results reveal that upregulation of miR-214 is a frequent event and negatively associated with PHLDA2 expression in osteosarcoma. Furthermore, miR-214 depletion enhanced apoptosis and radiosensitivity by inhibiting the Akt pathway via PHLDA2. The miR-214/PHLDA2/Akt axis provides a new avenue toward understanding the mechanism of radiosensitivity and may be a potential target for osteosarcoma intervention. Ethics Statement This study was carried out in accordance with the recommendations of international guidelines and ethical standards with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Institutional Ethics Committee of 920th Hospital of Joint Logistics Support Force, China. All animal experiments were approved by the Animal Care Committee of 920th Hospital of Joint Logistics Support Force, China. Author Contributions YL, XS, and HC conceived and designed the experiments. YL, YM, WM, MH, and BS performed the experiments. YL, ZL, QL, and YW analyzed the data. YL wrote the paper. HC, XS, and YL revised the paper. Funding This work was supported by the National Natural Science Foundation of China (Grant No.81572904 and 81560017). Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of int...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research