A Novel Citrullinated Modification of Histone 3 and Its Regulatory Mechanisms Related to IPO-38 Antibody-Labeled Protein
In this study we adopted an all-in-one method. Primers designed according to the two gRNA sequences and the tracRNA-U6 vector sequences were used to produce a gRNA1-tracRNA-U6-gRNA2 fragment. Then the proper fragment was ligated into the lentiCRISPRv2 vector and verified by sequencing. Lentiviral Packaging and Stable Cell Line Screening The constructed eukaryotic expression vector and gene knockdown vector were transfected into the 293T cells with the packaging plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher, Massachusetts, USA). The lentivirus was harvested 48 h after transfection, and the lentivirus supernatant was filtered using a 0.45 μm filter. One day prior to infection, the three cell lines (AGS, SGC7901, and MKN45) were plated at 2 × 105 cells per well in 6-well tissue culture plates. The lentivirus was added into the separate cell lines, and polybrene was added at a density of 6 ng/ul (Sigma, California, USA). After 24 h, the infection medium was removed and replaced with normal culture medium. After 48 h, the cell lines were screened using 2 ng/μl puromycin (Sangon Biotech, Shanghai, China), and a stable cell line was formed after 1 week of continuous selection. Western Blot Whole cellular protein was extracted using RIPA lysis buffer (Beyotime, Shanghai, China) containing a protease inhibitor cocktail (Roche, Basel, Switzerland). The cytoplasmic and nuclear protein fractions were isolated using a Nuclear and Cytoplasmic Protein Ex...
Authors: Pennisi PA, Fernández MC, Martin A Abstract Pheochromocytomas/paragangliomas (PCCs/PGLs) are rare neuroendocrine tumors, developed from chromaffin cells derived from the neural crest. From a genetic point of view PCCs/PGLs are divided as sporadic cases, and inherited cases as part of hereditary (familial) syndromes. While the majority is benign, up to 26% of PCCs/PGLs will undergo malignant transformation. Validated prognostic pathological parameters for malignant PCCs/PGLs are still lacking. Signaling that follows the interactions between IGFs and their receptor/s in tumor cells received extensive ...
Authors: Scheffer-Rath ME, Boot AM Abstract Vitamin D is important for skeletal growth, bone mineralization and dental health. Vitamin D deficiency is reported in many countries, may have several causes and can cause rickets in children. A guideline with recommendations of vitamin D supplementation to prevent nutritional rickets was published recently. The vitamin D receptor is present in many cells of the body including cells of the immune system. Many studies have been published on associations between vitamin D deficiency and extra skeletal effects, mainly without proof of causality in intervention studies. This...
Publication date: Available online 12 August 2020Source: Current Therapeutic ResearchAuthor(s): D.D. Gladman, A.M. Orbai, J. Gomez-Reino, S. Chang-Douglass, E. Leoncini, H.E. Burton, K.S. Kanik, A.B. Romero, J.C. Cappelleri, M-A. Hsu
The turmoil and devastating destruction of World War II had also ruined dermatology across Europe. In contrast, the Society of Investigative Dermatology (SID; founded in 1937) in the United States functioned successfully and soon became a role model for the leaders of dermatology departments in various European countries. Our former teachers in dermatology succeeded in overcoming the prejudice and animosity in the western world. This outcome was the conception of the founding fathers (there was no founding lady in the initial group) of the European Society for Dermatological Research (ES DR).
The European Society for Dermatological Research (ESDR) has been my home in skin research since the mid-1980s when I started training as a dermatology resident and physician scientist in Z ürich, Switzerland. After active research contributions to the annual meetings for many years, I became a member of the ESDR board and then Secretary Treasurer from 1999 to 2001, followed by the ESDR presidency in 2002. Characteristic for my time in the ESDR leadership was pursuit of scientific exc ellence at the meetings, international growth, and intersociety collaboration.
On behalf of all the Japanese Society for Investigative Dermatology (JSID) members, we would like to congratulate the European Society for Dermatological Research (ESDR) on its 50th anniversary! It must have been a long and winding road to achieve the current big success of ESDR, which always provides a harmonious and welcoming atmosphere to people attending its meetings from around the world. Although each country in European communities has a different background, ESDR unites people with the same aim to advance investigative dermatology and cutaneous biology.
Having been involved in the European Society for Dermatological Research (ESDR) from the 1980s, we have had the opportunity to observe the spectacular development of our society from early small meetings in the Netherlands to major international meetings held in many European centers. Our first ESDR meetings were notable because of the stimulating and friendly atmosphere with full sharing of ideas and new discoveries, even prepublication. Such an atmosphere has been preserved over time and has generated many research partnerships, including our own collaboration in keratinocyte (KC) biology and genetic skin diseases.
To better understand and interpret the trends in cutaneous research, we carried out a network analysis of all the titles of the submitted abstracts of the annual meetings of the European Society of Dermatological Research (ESDR), including the International Investigative Dermatology (IID) meetings between 2010 and 2019. Network analysis is a data science tool to process, analyze, and visualize big sets of data. As expected, psoriasis was the frontrunner in each of the annual meetings, followed by dermatitis and melanoma.
Authors: Guo FF, Jiang MM, Hong LL, Qiao B, Lin XM, Xu WY, Fu XQ Abstract Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long non-coding RNA OR3A4 promotes metastasis of ovarian cancer via inhibiting KLF6, by F.-F. Guo, M.-M. Jiang, L.-L. Hong, B. Qiao, X.-M. Lin, W.-Y. Xu, X.-Q. Fu, published in Eur Rev Med Pharmacol Sci 2019; 23 (6): 2360-2365-DOI: 10.26355/eurrev_201903_17380-PMID: 30964160" has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https://www.e...
CONCLUSIONS: The study does not seem to exclude that the lethality of COVID-19 may be climate sensitive. Future studies will have to confirm these clues, due to potential confounding factors, such as pollution, population age, and exposure to malaria. PMID: 32767354 [PubMed - in process]
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