A Novel Citrullinated Modification of Histone 3 and Its Regulatory Mechanisms Related to IPO-38 Antibody-Labeled Protein

In this study we adopted an all-in-one method. Primers designed according to the two gRNA sequences and the tracRNA-U6 vector sequences were used to produce a gRNA1-tracRNA-U6-gRNA2 fragment. Then the proper fragment was ligated into the lentiCRISPRv2 vector and verified by sequencing. Lentiviral Packaging and Stable Cell Line Screening The constructed eukaryotic expression vector and gene knockdown vector were transfected into the 293T cells with the packaging plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher, Massachusetts, USA). The lentivirus was harvested 48 h after transfection, and the lentivirus supernatant was filtered using a 0.45 μm filter. One day prior to infection, the three cell lines (AGS, SGC7901, and MKN45) were plated at 2 × 105 cells per well in 6-well tissue culture plates. The lentivirus was added into the separate cell lines, and polybrene was added at a density of 6 ng/ul (Sigma, California, USA). After 24 h, the infection medium was removed and replaced with normal culture medium. After 48 h, the cell lines were screened using 2 ng/μl puromycin (Sangon Biotech, Shanghai, China), and a stable cell line was formed after 1 week of continuous selection. Western Blot Whole cellular protein was extracted using RIPA lysis buffer (Beyotime, Shanghai, China) containing a protease inhibitor cocktail (Roche, Basel, Switzerland). The cytoplasmic and nuclear protein fractions were isolated using a Nuclear and Cytoplasmic Protein Ex...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

Related Links:

ABSTRACT: As the North American population of older adults increases, so does the need for nursing students to gain skills in geriatric care. Locating appropriate clinical sites for undergraduate students, including community and health promotion opportunities, prompted a public university's school of nursing to partner with a faith-based respite program. The collaboration between a public educational institution and a church-based program benefited the geriatric participants, the program leadership, and the nursing students and faculty.
Source: Journal of Christian Nursing - Category: Nursing Tags: JCN Online Extra: online only: education Source Type: research
Conclusions: Our study suggests that greater attention to the experience and interpersonal relationships of dyads has the potential for improving HF self-management and facilitating patient-centered care.
Source: Journal of Cardiovascular Nursing - Category: Nursing Tags: ARTICLES: Heart Failure Source Type: research
Conclusions: Thirst in patients with HF was related to a higher dose of loop diuretics and fluid restriction. Healthcare providers should realize that it is important to assess thirst regularly and reconsider the need of a fluid restriction and the amount of loop diuretics in case of thirst.
Source: Journal of Cardiovascular Nursing - Category: Nursing Tags: ARTICLES: Heart Failure Source Type: research
Conclusions The older patient with HF should be carefully assessed for nonadherence. The development of interventions that are patient specific, target medications with the greatest potential for nonadherence, and use easy-to-access resources may promote decisions for medication adherence. More research is needed to develop interventions that promote decisions for medication adherence.
Source: Journal of Cardiovascular Nursing - Category: Nursing Tags: ARTICLES: Heart Failure Source Type: research
Conclusions: There is a need to develop and test interventions that improve self-management in elderly patients with hypertension. Specifically, individualized interventions to promote exercise among elderly persons with hypertension who are single and living alone are needed. Male patients with a lower education level, poor well-being, poor family function, and the lowest self-management levels are a key population to target.
Source: Journal of Cardiovascular Nursing - Category: Nursing Tags: ARTICLES: Cardiovascular Disease Source Type: research
Conclusions Findings from this study suggest that SBML is a natural fit for the high-risk tasks needed to save VAD self-care. The 7 unique training-related themes derived from the qualitative data informed the design and development of a VAD SBML self-care curriculum.
Source: Journal of Cardiovascular Nursing - Category: Nursing Tags: ARTICLES: Cardiovascular Disease Source Type: research
No single product is responsible for the thousands of vaping-related lung illnesses reported across the United States, according to the US Centers for Disease Control and Prevention. The CDC has reported 2,291 confirmed cases of vape-related lung injury.
Source: CNN.com - Health - Category: Consumer Health News Source Type: news
We present a case of a 58-year-old man, with the typical clinical aspect of PRP with a four-month onset of the disease. The histopathological and dermatoscopical findings confirmed the PRP diagnosis. The routine laboratory results were in normal limits, except the number of eosinophils, which was elevated and the number of lymphocytes, which was lower. After a thorough examination within a hematological consultation, the cause of hypereosinophilia remained unknown. An imagistic examination was performed and a prostate hypertrophy was noted. The prostate-specific antigen (PSA) level was found to be increased. The urologic c...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
In conclusion, the particular mode of intervention by which ERK1/2 might influence cell proliferation and/or apoptosis processes depends on the type of therapeutic agent, the cells' particularities, and the activation status of the ERK1/2. PMID: 31798724 [PubMed]
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
Authors: Bratu OG, Diaconu CC, Mischianu DLD, Constantin T, Stanescu AMA, Bungau SG, Ionita-Radu F, Marcu RD Abstract Prostate cancer is the second most common form of cancer in men in Europe. The primary treatment of this type of cancer is radical prostatectomy, which has shown good oncological results. Radical prostatectomy (open, laparoscopic or robotic) has high success and low morbidity rates in patients with localized prostate cancer. The life expectancy is>10 years after radical prostatectomy. Studies have shown that ~20%-30% of the patients who have undergone radical prostatectomy can develop biochemical...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
More News: Academia | Academies | Adenocarcinoma | Arthritis | Autoimmune Disease | Biology | Biotechnology | Brain | Breast Cancer | Cancer | Cancer & Oncology | Cardiology | Chemistry | China Health | Colorectal Cancer | Databases & Libraries | Epithelial Cancer | Funding | Gastric (Stomach) Cancer | Gastroenterology | Genetics | Heart | Heart Failure | Hospitals | Japan Health | Laboratory Medicine | Leukemia | Liver | Lymphoma | Melanoma | Men | Molecular Biology | Myeloma | Nanotechnology | Netherlands Health | Neurology | Neuroscience | Neurosurgery | Oral Cancer | Ovarian Cancer | Ovaries | Prostate Cancer | Reproduction Medicine | Rheumatoid Arthritis | Rheumatology | Science | Skin Cancer | Sodium | Statistics | Stem Cell Therapy | Stem Cells | Students | Study | Switzerland Health | Translocation | Universities & Medical Training | Urology & Nephrology | USA Health | Websites