Effects of Crinum jagus Water/Ethanol Extract on Shigella flexneri-Induced Diarrhea in Rats.

This study aimed to evaluate anti-Shigella flexneri activities of Crinum jagus water/ethanol extract. In vitro activities were assayed by disc diffusion and agar dilution methods and in vivo section on Shigella flexneri-induced diarrhea in rats. This was done by oral administration of 9 X 108 CFU of Shigella flexneri to rats that were treated twice daily with Crinum jagus water/ethanol extract for seven consecutive days. Ciprofloxacin was used as positive control. Daily Shigella flexneri load was evaluated. After one treatment week, animals were then sacrificed and interleukins (IL-2 and INF-γ), immunoglobulins (IgA and IgM), motilin, vasoactive intestinal peptide, and ions (sodium, potassium, calcium, and chloride) levels were determined. Also, blood cell count was realized. Crinum jagus water/ethanol extract dose-dependently inhibited Shigella flexneri growth with inhibition diameter of 18.90 and 25.36 mm, respectively, at 0.39 and 200 mg/mL. Minimum inhibitory concentration (MIC) was 0.10 mg/mL and minimum bactericidal concentration (MBC) was 0.30 mg/mL with MBC/MIC ratio of 3.0. In Shigella flexneri-induced diarrheic rats, Crinum jagus reduced (p<0.01) diarrheal stools emission and Shigella load and lowered IL-2, INF-γ, IgA, IgM, and motilin blood levels, whereas it increased (p<0.01) vasoactive intestinal peptide, sodium, potassium, calcium, and chloride blood levels. In diarrheal rats, Crinum jagus restored the decreasing white blood cells and haemoglobi...
Source: Methods of Information in Medicine - Category: Information Technology Authors: Tags: Evid Based Complement Alternat Med Source Type: research