Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis

In this study, eight phages were sequentially separated from sewage (Table 2). Phages vB_EcoS_IME18 (IME18), vB_EcoS_IME253 (IME253), vB_EcoM_IME281 (IME281), vB_EcoM_IME338 (IME338), vB_EcoM_IME339 (IME339), vB_EcoM_IME340 (IME340), vB_EcoM_IME341 (IME341), and vB_EcoS_IME347 (IME347) formed clear, bright plaques on soft agar plates (Supplementary Figure S2). TEM imaging showed that phages IME281 (Figure 1), IME339 (Figure 2), IME340 (Figure 3), and IME341 (Figure 4) had a prolate head and a tail with a contractile sheath. The elongated icosahedral head was ∼100 nm long and 80 nm wide, while the tail tube was ∼100 nm long. Phage IME338 (Figure 5) had an icosahedral head approximately 60 nm wide and a tail with a contractile sheath approximately 100 nm long. Phages IME18 (Figure 6), IME253 (Figure 7) and IME347 (Figure 8) had an icosahedral head and a non-contractile tail, with a head diameter of approximately 60 nm and a tail length of approximately 160 nm. TABLE 2 Table 2. Phages isolated in this study. FIGURE 1 Figure 1. Transmission electron micrograph images of Escherichia coli phage vB_EcoM_IME281. FIGURE 2 Figure 2. Transmission electron micrograph images of Escherichia coli phage vB_EcoM_IME339. FIGURE 3 Figure 3. Transmission electron micrograph images of Escherichia coli phage vB_EcoM_IME340. FIGURE 4 Figure 4. Transmission electron micrograph images of Escherichia coli phage vB_EcoM_IME341. ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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