Large-Scale Genomic Analyses and Toxinotyping of Clostridium perfringens Implicated in Foodborne Outbreaks in France

This study provides new insight into the characterization of foodborne C. perfringens and highlights the potential of WGS for the investigation of FBOs. Introduction Clostridium perfringens is a Gram-positive, spore-forming, anaerobic, rod-shaped bacterium, known as an important causative agent of foodborne and non-foodborne gastroenteritis (Grass et al., 2013). The ability of this bacterium to form resistant spores contributes to its survival in many environmental niches, including soil, sewage, foods, and the intestinal microbiota of humans and animals (Xiao, 2014; Li et al., 2016). C. perfringens can cause necrotic enteritis, enterotoxemia, and gastroenteritis in animals (Keyburn et al., 2008; Uzal et al., 2015). It is also a foodborne pathogen that causes diarrhea and abdominal pain in humans (Scallan et al., 2011). C. perfringens is commonly found as a contaminant in meat and poultry products, as well as in vegetables and crops. This bacterium is an important pathogen involved in foodborne outbreaks (FBOs) and ranks as the fourth most common causative agent of foodborne illness due to bacterial toxins in France and Europe, with more than 1,500 human cases each year (EFSA, 2016). The pathogenicity of this bacterium is largely attributable to its ability to produce a variety of virulence factors. These virulence factors include well-characterized pathogenic toxins and hydrolytic enzymes. Their roles in FBO remain to be defined but their presence is an aggravating f...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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This study aimed to investigate the risk of self-harm following diagnosis of different psychiatric disorders in an Asian population, through a review of inpatient records retrieved from the Hong Kong Clinical Data Analysis and Reporting System (CDARS).MethodsFor this nested case-control study, the inpatient data of people admitted for any reason to Hong Kong public hospitals, between Jan 1, 2000, and Dec 31, 2010, were extracted from CDARS. Cases were people aged 10 years or older who had been admitted to inpatient care with a first-listed record of psychiatric disorder during the study period. The seven psychiatric disord...
Source: The Lancet Psychiatry - Category: Psychiatry Source Type: research
In conclusion, the addition of PPIs to the treatment regimen of breast cancer appears to be a promising strategy to potentiate the efficacy of chemotherapy and may suppress cancer metastasis. PMID: 31955147 [PubMed - in process]
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Abstract A series of nine new 2,3-disubstituted 4(3H)-quinazolin-4-one derivatives was furnished starting from the 2-propyl-4(3H)-quinazo-line-4-one (2). The reinvestigation of the key starting quinazolinone 2 was performed under microwave irradiation (MW) and solvent-free conditions. Combination of MW and phase-transfer catalysis using tetrabutylammonium benzoate (TBAB) as a novel neutral ionic catalyst was used for carrying out N-alkylation and condensation reactions of compound 2 as a simple, efficient and eco-friendly technique. The structure of the synthesized compounds was elucidated using different spectral...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
This study aims to unveil the mechanisms underlying the pro-apoptotic effect of Cur-NLC on HepG2 cells. Evidence indicates that low expression of death receptors (DRs) on cancer cell membranes leads to attenuated apoptosis signaling. This study showed that Cur-NLC significantly increased total expression of DR5 protein while simultaneously upregulated cell membrane expression of DR5. Cur-NLC significantly increased caspase-8 and caspase-3 activities, accompanied by increased apoptosis. Furthermore, enhanced apoptosis was inhibited in the presence of a pan-caspase inhibitor, Z-VAD-FMK. Therefore, Cur-NLC induced activation ...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Abstract The incidence of mortality of prostate cancer (PCa) has been an uptrend in recent years. Our previous study showed that the sex-determining region Y-box 7 (SOX7) was low-expressed and served as a tumor suppressor in PCa cells. Here, we describe the effects of polyphyllin D (PD) on proliferation and cell cycle modifications of PCa cells, and whether SOX7 participates in this process. PC-3 cells were cultured in complete medium containing PD for 12, 24, and 48 h. MTT assay was used to investigate the cytotoxic effects of PD. Cell cycle progression was analyzed using propidium iodide (PI) staining, and prote...
Source: Acta Pharmaceutica - Category: Drugs & Pharmacology Authors: Tags: Acta Pharm Source Type: research
Conclusion: SliceTracker is modular and extensible open source platform for supporting image processing aspects of the tpMRgBx procedure. It has been successfully utilized to support clinical research procedures at our site.
Source: IEEE Transactions on Biomedical Engineering - Category: Biomedical Engineering Source Type: research
Publication date: Available online 20 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Alicia Gutiérrez, Lorena Sambuco, Laura Álvarez, Mariel Núñez, Rosa Bergoc, Elsa Zotta, Gabriela Martín, Andrea RandiAbstractBreast cancer is currently the leading cause of cancer death among women worldwide. AP-1 (c-Fos/c-Jun) is associated with proliferation and survival, while cytoplasmic c-Fos activates phospholipid synthesis in cells induced to differentiate or grow. Estrogen receptor α 46 (ERα46) is a splice variant of full-length ERα66 and ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
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Source: International Journal of Environmental Analytical Chemistry - Category: Chemistry Authors: Source Type: research
Phys. Chem. Chem. Phys., 2020, Accepted Manuscript DOI: 10.1039/C9CP06530J, PaperPablo Abreu Morais, Francisco Francin é Maia, Jr., Christian Solis-Calero, Ewerton Wagner Santos Caetano, Valder Nogueira Freire, Hernandes Faustino Carvalho Despite being recognized as a therapeutic target in the processes of cancer cell proliferation and metastasis for over 50 years, the interaction of the urokinase plasminogen activator uPA with its... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Phys. Chem. Chem. Phys. latest articles - Category: Chemistry Authors: Source Type: research
Publication date: Available online 20 January 2020Source: Journal of Health EconomicsAuthor(s): Corrado Giulietti, Mirco Tonin, Michael VlassopoulosAbstractThis paper provides evidence that daily fluctuations in the stock market have important–and hitherto neglected–spillover effects on fatal car accidents. Using the universe of fatal car accidents in the United States from 1990 to 2015, we find that a one standard deviation reduction in daily stock market returns is associated with a 0.6% increase in fatal car accidents that happen after the stock market opening. A battery of falsification tests support a caus...
Source: Journal of Health Economics - Category: Health Management Source Type: research
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