Lipopolysaccharide Metabolism and Identification of Potential Biomarkers Predictive of Graft-versus-host Disease After Allogeneic Stem Cell Transplantation

Conditions:   Hematologic Diseases;   Stem Cell Transplantation Intervention:   Other: Blood sample Sponsor:   Centre Hospitalier Universitaire de Besancon Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials

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In the 1960s and 70s, eradication of host bacteria was thought to be achievable and beneficial to avoiding infection in the immunocompromised host. At that time, patients were fed autoclaved, sterile foods and kept in totally protected environments1. Over time, there became an obvious need to liberalize dietary options in these patients as the sterile foods were not palatable, nor did they provide enough variety [1]. The neutropenic diet was invented to meet this need and essentially eliminates certain food groups, most commonly raw fruits and vegetables, which contain bacteria, in an effort to decrease bacterial content a...
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
Publication date: Available online 29 April 2019Source: Clinical Lymphoma Myeloma and LeukemiaAuthor(s): Yaohua Cao, Ning Wan, Zhuoru Liang, Jingmei Xie, Sen Wang, Tengfei Lin, Tiantian Zhang, Jie JiangAbstractMany new regimens have been applied to newly diagnosed transplant-ineligible multiple myeloma, but no head-to-head research has been performed to compare the efficacy of these treatments. Currently lenalidomide plus dexamethasone (Rd) is one of the standard treatments. Our aim was to make a comparison of these treatments to Rd by a network meta-analysis. We performed a systematic review and network meta-analysis. We ...
Source: Clinical Lymphoma Myeloma and Leukemia - Category: Cancer & Oncology Source Type: research
Publication date: Available online 23 May 2019Source: Stem Cell ReportsAuthor(s): Maher Hanoun, Anna Arnal-Estapé, Maria Maryanovich, Ali H. Zahalka, Sarah K. Bergren, Chee W. Chua, Avigdor Leftin, Patrik N. Brodin, Michael M. Shen, Chandan Guha, Paul S. FrenetteSummaryIn the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation,...
Source: Stem Cell Reports - Category: Stem Cells Source Type: research
ConclusionWe have used MSC-exo via intranasal administration to treat both BTBR and shank3 KO mice. The behavioral effect seemed 3 weeks post the treatment.The behavioral amelioration was seen in all the core symptoms including social interaction, repetitive behaviors, and vocal communication.
Source: Cytotherapy - Category: Cytology Source Type: research
ConclusionIn this study, we assessed the feasibility of intranasal delivery of MSCs-derived EVs to improve the positive and negative symptoms in a PCP model of schizophrenia. Our results show that mice treated with MSCs-EVs are beneficial for preventing both positive and negative symptoms in a PCP-model of schizophrenia. While PCP-treated mice showed a deficiency in social interaction and increased vulnerability to amphetamine, mice co-administered with PCP and MSCs-EVs behaved similarly to the sham group, treated with saline. Immunohistochemical studies demonstrated that the EVs prevented loss of Paravalbumin-expressing G...
Source: Cytotherapy - Category: Cytology Source Type: research
ConclusionFor details of pre/post processing, see Table. Since early 2017 we introduced chest/abdominal cavity wash with 1:1 saline-betadine mix and scrubbing T11-L4VB logs with chlora-prep that has eliminated bacterial contamination for the last 3 products. The first 2 were infused without adverse events following IRB/FDA approved antibiotic prophylaxis.Initially, processing of the VBs was arduous, using a scalpel and wire brush to clean the logs, then dissecting into small pieces with a mallet and osteotome. Since early 2017 a Stryker RemB saw has been used. A physical task that had taken 2 technologists 3 hours to compl...
Source: Cytotherapy - Category: Cytology Source Type: research
Conclusion Study DesignThis is a phase I/II clinical study involving patients who underwent an allogeneic HSCT for malignant or non malignant haematological disorders and developed grade II-IV acute GVHD. A total of 40 eligible patients will be recruited in this study.For Phase I open labelled study, 5 eligible patients will be recruited to receive Cyto-MSC (5 × 106 MSC per kg bodyweight) and standard treatment. Meanwhile, for Phase II double blinded placebo controlled study, another 35 eligible patients will be recruited and randomized into 2 study groups where 15 patients will be assigned into Group A to receiv...
Source: Cytotherapy - Category: Cytology Source Type: research
ConclusionThe dosing for this indication is likely to be in the order of 100 million cells per infusion. Large numbers of cells thus need to be manufactured and using standard cell culture techniques would make it too expensive and labour intensive. A standard expansion protocol in flasks or cell factories is an open process, and therefore run in expensive clean rooms to avoid the risk of contamination. We have explored several state-of-the art-bioreactor systems, and various types of microcarriers in stirred tanks from different manufacturers. We have tested these systems to optimize the complete workflow of HepaStem cult...
Source: Cytotherapy - Category: Cytology Source Type: research
ConclusionBoth of UC-MSCs and SVF were divided into 4 groups with two variables temperature and medium storage. Cells have been evaluated in room temperature (RT) (18-22°C) and cold temperature (2-8°C) with 0.9% NaCl and conditioned medium (UC-MSCs) or autologous serum (SVF). The viability from each group was assessed day by day using Tryphan Blue.At room temperature, viability of cells in both storage mediums ware significantly decreased while stored at cold temperature the viability was more stable. The highest viability of UC-MSCs at RT was obtained from cells in 0.9% NaCl solution (77% viable). However, the hig...
Source: Cytotherapy - Category: Cytology Source Type: research
Conclusion MethodsThis study was conducted on 18 eyes of 18 volunteers with retinitis pigmentosa, The cells delivery with peribulbar injection method. Clinical examinitation, visus and fundus examination, multifocal electroretinography were performed before and after an peribulbar injection of approximately one-million UC-MSC. The patients were followed in 1 hour, 24 hours and 1 month for safety evaluation.Results: There are adverse events, like edema under the eyes, were observed in eyes all patients 1 hour after transplantation of UC-MSCs. The edema will disappear within 24 hours. These patients reported improvements in ...
Source: Cytotherapy - Category: Cytology Source Type: research
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