Toxic, cytogenetic and antitumor evaluations of [6]-gingerol in non-clinical in vitro studies

Publication date: July 2019Source: Biomedicine & Pharmacotherapy, Volume 115Author(s): Rosália Maria Tôrres de Lima, Antonielly Campinho dos Reis, José Victor de Oliveira Santos, José Roberto de Oliveira Ferreira, Antonio Lima Braga, José Williams Gomes de Oliveira Filho, Ag-Anne Pereira Melo de Menezes, Ana Maria Oliveira Ferreira da Mata, Marcus Vinícius Oliveira Barros de Alencar, Debora Caroline do Nascimento Rodrigues, Paulo Michel Pinheiro Ferreira, Teresinha de Jesus Aguiar dos Santos Andrade, Juan Carlos Ramos Gonçalves, Felipe Cavalcanti Carneiro da Silva, João Marcelo de Castro e Sousa, Ana Amélia de Carvalho Melo CavalcanteAbstractGingerol - [6]-gingerol ((S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone; [6]-G) - is a phenolic compound with several pharmacological properties. Herein, the aim of the study was to evaluate the toxicogenic effects of [6]-G on Artemia salina nauplii, Allium cepa, HL-60 cell line and Sarcoma 180 (S-180) ascitic fluid cells.For toxic and genotoxic analysis, it was used [6]-G concentrations of 5, 10, 20 and 40 μg mL-1. For cytotoxic evaluation using the MTT test (3- [4,5-dimethyl-thiazol-2-yl] -2,5-diphenyl tetrazolium bromide), serial [6]-G dilutions (1.56–100 μg mL-1) were performed, and S-180, HL-60 and peripheral blood mononuclear cells (PBMC) were treated for 72 h. The IC50 of [6]-G were 1.14, 5.73 and 11.18 μg mL-1 for HL-60, S-180 and PBMC, respectively, indicating a possible selectivity against tum...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research