Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy
In this study, all tumors were adenocarcinomas that originate from ducts or ductules but infiltrate to involve all components of the pancreas. Other tumors that originate in pancreas may have different properties and a future study of in situ tumor is likely needed to determine whether the tumor tissue retains a structural remnant of its originating tissue. Methods and Materials Histology Sample Preparation Samples of normal human pancreas from five patients and pancreatic ductal adenocarcinoma tissue samples from ten patients were obtained with informed consent and institutional approval (University Health Network Toronto, Canada). The tissues were handled as per standard clinical histology protocols. Thin sections (5 μm) were cut from formalin-fixed paraffin-embedded tissues, mounted on glass slides and stained with H&E for histopathologic analysis. All slides were scanned at ×20 magnification using a whole-slide scanner (ScanScope XT: Leica Biosystems, Germany). In each slide, 110 × 110 μm regions of interest, as identified by a pathologist (S.L.A), were scanned. Identification was performed by assessing the tissue architecture and cytology of the tumor cells in the high resolution bright-field microscopy images. A total of 47 tumor and 51 normal regions were imaged to determine quantitative differences between tumor and normal tissue. Two-tailed t-tests of statistical significance were performed. Non-linear Optical Microscope Setup A custom-built Y...
According to a study published inThe Journal of Clinical Endocrinology& Metabolism, obesity is not associated with an increased risk of developing thyroid disease or thyroid cancer.Endocrinology Advisor
According to a study published in theInternational Journal of Cancer, light pollution at night time may increase breast cancer risk by 10% in post-menopausal women.inews
Authors: Miceli A, Raffa S, Donegani MI, Cossu V, Morbelli S, Marini C, Sambuceti G, Bauckneht M Abstract Sarcoidosis is a systemic inflammatory disease of unknown etiology, pathologically characterized by non-caseating granulomas involving several organs and tissues. This pathological process can eventually affect the heart during his course leading to fibrosis associated with systolic dysfunction, conduction disturbance, and even sudden cardiac death. Due to this prognostic impact, diagnosis is crucial to optimize clinical management. The low sensitivity of endomyocardial biopsy and its invasive nature prevents i...
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