Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy

In this study, all tumors were adenocarcinomas that originate from ducts or ductules but infiltrate to involve all components of the pancreas. Other tumors that originate in pancreas may have different properties and a future study of in situ tumor is likely needed to determine whether the tumor tissue retains a structural remnant of its originating tissue. Methods and Materials Histology Sample Preparation Samples of normal human pancreas from five patients and pancreatic ductal adenocarcinoma tissue samples from ten patients were obtained with informed consent and institutional approval (University Health Network Toronto, Canada). The tissues were handled as per standard clinical histology protocols. Thin sections (5 μm) were cut from formalin-fixed paraffin-embedded tissues, mounted on glass slides and stained with H&E for histopathologic analysis. All slides were scanned at ×20 magnification using a whole-slide scanner (ScanScope XT: Leica Biosystems, Germany). In each slide, 110 × 110 μm regions of interest, as identified by a pathologist (S.L.A), were scanned. Identification was performed by assessing the tissue architecture and cytology of the tumor cells in the high resolution bright-field microscopy images. A total of 47 tumor and 51 normal regions were imaged to determine quantitative differences between tumor and normal tissue. Two-tailed t-tests of statistical significance were performed. Non-linear Optical Microscope Setup A custom-built Y...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

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