RNAseq Profiling of Leukocyte Populations in Zebrafish Larvae Reveals a cxcl11 Chemokine Gene as a Marker of Macrophage Polarization During Mycobacterial Infection

In conclusion, the transcriptome analyses reported here present a unique and detailed genetic profile of zebrafish larval immune cells, thereby providing a valuable resource that can be data mined to verify the expression of specific genes in the profiled cell types or to identify novel genes of interest and potential cell-specific markers. In future work single cell RNA sequencing technology will be useful to interrogate the heterogeneity in expression profiles of resting and activated macrophages. Ethics Statement This study was carried out in accordance with the local animal welfare regulations as overseen by the Animal Welfare Body of Leiden University (License number: 10612). The protocol was approved by the international guidelines specified by the EU Animal Protection Directive 2010/63/EU. Author Contributions JR contributed to conception and design of the study, performed and analyzed experiments, and wrote and edited the manuscript. VT contributed to conception and design of the study, performed and analyzed experiments, and wrote and edited a section of the manuscript. AZ and ZK contributed to conception and design of the study and performed experiments. HJ performed and analyzed experiments. FS performed experiments. AM contributed to conception and design of the study, wrote and edited the manuscript, and acquired funding. All authors contributed to manuscript revision, read and approved the submitted version. Conflict of Interest Statement HJ was employed b...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research