Combination of clotam and vincristine enhances anti-proliferative effect in medulloblastoma cells.

Combination of clotam and vincristine enhances anti-proliferative effect in medulloblastoma cells. Gene. 2019 Apr 13;: Authors: Patil S, Sankpal UT, Hurtado M, Bowman WP, Murray J, Borgmann K, Ghorpade A, Sutphin R, Eslin D, Basha R Abstract Medulloblastoma (MB) is characterized by highly invasive embryonal neuro-epithelial tumors that metastasize via cerebrospinal fluid. MB is difficult to treat and the chemotherapy is associated with significant toxicities and potential long-term disabilities. Previously, we showed that small molecule, clotam (tolfenamic acid: TA) inhibited MB cell proliferation and tumor growth in mice by targeting, survivin. Overexpression of survivin is associated with aggressiveness and poor prognosis in several cancers, including MB. The aim of this study was to test combination treatment involving Vincristine® (VCR), a standard chemotherapeutic drug for MB and TA against MB cells. DAOY and D283 MB cells were treated with 10 μg/mL TA or VCR (DAOY: 2 ng/mL; D283: 1 ng/mL) or combination (TA + VCR). These optimized doses were lower than individual IC50 values. The effect of single or combination treatment on cell viability (CellTiterGlo kit), Combination Index (Chou-Talalay method based on median-drug effect analysis), activation of apoptosis and cell cycle modulation (by flow cytometry using Annexin V and propidium iodide respectively) and the expression of associated markers including survivin (Wes...
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research