The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery
In this study, we attempted to find a DNA extraction protocol which could be effectively used to analyze both the bacterial and fungal community. We evaluated the effect of five DNA extraction methods (QIAamp DNA Stool Mini Kit, PureLinkTM Microbiome DNA Purification Kit, ZR Fecal DNA MiniPrepTM Kit, NucleoSpin® DNA Stool Kit, and IHMS protocol Q) on bacterial and fungal gut microbiome recovery using (i) a defined system of germ-free mice feces spiked with bacterial or fungal strains, and (ii) non-spiked human feces. In our experimental setup, we confirmed that the examined methods significantly differed in efficiency and quality, which affected the identified stool microbiome composition. In addition, our results indicated that fungal DNA extraction might be prone to be affected by reagent/kit contamination, and thus an appropriate blank control should be included in mycobiome research. Overall, standardized IHMS protocol Q, recommended by the International Human Microbiome Consortium, performed the best when considering all the parameters analyzed, and thus could be applied not only in bacterial, but also in fungal microbiome research. Introduction The human gut microbiome has been a subject of study for more than a century (Mattill and Hawk, 1911; Rogers et al., 1918), but with the recent advent of new culture-independent technologies (i.e., massive parallel sequencing), it has attracted enormous attention. Numerous researchers have described its association wi...
Thousands of Covid-19 patients have been treated with blood plasma outside of rigorous clinical trials — hampering research that would have shown whether the therapy worked.
Weight-bearing CT scans can provide new insight into foot and ankle disorders and valuable information for surgical planning.Journal of the American Academy of Orthopaedic Surgeons
Vaccines protecting against influenza, hepatitis B and rabies are less effective for obese people. As scientists develop a Covid-19 vaccine, experts say obesity could be an impediment, a sobering prospect for the US, where nearly half of all adults are obese.
Stacey Nagy told CNN's Erin Burnett she wrote an obituary for her husband's coronavirus death where she blamed President Trump, Texas Gov. Abbott and those who do not wear masks because she felt a need to "put blame where it belonged."
The cross-party inquiry is highly critical of the UK Government's 'inexplicable' decision to lift its initial quarantine measures in mid-March, ten days before lockdown.
SUPPLEMENTS could be used to improve the health of your heart, as well as to protect against high blood pressure symptoms and signs. Add these cheap garlic capsules to your diet to lower your risk of heart problems, including heart attacks and heart disease.
Advice and information on how to look after your mental health and wellbeing during the coronavirus (COVID-19) outbreak. Update in this version (published 4th August 2020) - added translated documents in Arabic, Bengali, Chinese CN, Chinese HK, French, Gujarati, Polish, Portuguese, Punjabi and Urdu.
This is advice for parents and carers on looking after the mental health and wellbeing of children or young people during the coronavirus (COVID-19) outbreak. Update in this version (published 4th August 2020) - added translated documents in: Arabic, Bengali, Chinese CN, Chinese HK, French, Gujarati, Polish, Portuguese, Punjabi and Urdu.
Those suffering from cancer, heart conditions, strokes, diabetes and lung disease are among the worst affected by delays to their diagnosis and care, a survey of senior doctors reveals.
HOW TO live longer: Long life expectancy is something we would all like to strive for. According to studies, the timing of when you eat could be just as important as what you eat. Health experts advise a new way of eating which will help burn fat, improve memory, aid in a healthier heart, reduce the risk of disease and boost longevity.
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